胃癌组织中PTEN与COX-2基因表达及临床意义

目的检测PTEN、COX-2在胃癌组织中的表达情况,探讨其与胃癌发生发展的关系及作用机理。方法用免疫组化S-P法检测胃癌组织与非胃癌黏膜中PTEN与COX-2表达情况。结果 80例胃癌组织中42例PTEN蛋白表达明显下降或缺失,且与胃癌TNM分期、淋巴结转移相关(P<0.05)。COX-2在胃癌组织中的阳性率为67.5%,显著高于非胃癌黏膜组织(P<0.05),并相关于胃癌淋巴结转移、TNM分期。结论抑癌基因PTEN与凋亡抑制基因COX-2可能存在相互作用,共同参与了胃癌的发展、转移及浸润。

Expression and clinical significance of genes PTEN and COX-2 in gastric cancer tissue

Objective To detect the expression of genes PTEN and COX-2 in gastric carcinoma tissue, and investigate the relationship between both genes and their clinical significances in tumorigenesis and progression of gastric carcinoma. Methods The expression of PTEN and COX-2 were detected by S-P immunohistochemistry methods. Results PTEN protein was found reduced or absent in 42 cases of gastric cancers. There was statistically significant correlation between nuclear PTEN expression and TNM staging, lymphnote metastasis(P<0.05). The COX-2 expression in gastric carcinoma was higher than the normal gastric mucosa (P<0.05), and was related statistically with lymphote metastasis and TNM staging (P<0.05). Conclusion There is possible interaction between PTEN and COX-2, which may be contribute in the tumorigenesis, growth and metastasis of gastric carcinoma.