近交系大鼠DA到LEW肝移植急性排斥模型的特点

目的 探讨近交系大鼠DA到LEW原位肝移植模型的特点并判断排斥反应发生的强度.方法 采用Kamada二袖套法,利用封闭群大鼠SD和Wistar进行建模技能训练,在此基础上建立近交系大鼠DA到LEW肝移植模型,根据临床表现和Banff标准判断排斥反应发生的强度.结果 共施行DA到LEW大鼠肝移植模型15例,手术成功率86.7%,死亡原因为肝上下腔静脉漏血、肝下下腔静脉血栓、胆道梗阻.术后第3天开始出现排斥反应,7 d后逐渐达到高峰,除术后并发症致死外,其余均在12 d内死于Ⅲ级排斥反应.结论 DA到LEW为稳定、强烈的大鼠肝移植急排模型,是研究肝移植排斥反应及免疫耐受的理想动物模型.但近交系大鼠在组织结构上有其自身特点,给建模带来一定难度.

Characteristics of acute rejection model of DA to LEW orthotopic liver transplantation in inbred rats

Objective To study the characteristics of liver transplantation models in inbred rats DA to LEW and judge the grades of acute rejection. Methods Surgical skills were trained by using closed colony SD and Wistar rats, followed by DA to LEW orthotopic liver transplantation with Kama-da's two-cuff technique. The grades of acute rejection were judged by their clinical manifestations and Banff schema. Results Fifteen models of DA to LEW liver transplantation were established. The suc-cessful rate was 86.7 %. The causes of death were hemorrhage and thrombosis of suprahepatic and in-frahepatic vena cava and obstruction of biliary tract. Grade I rejection occurred on the 3rd d and reached the peak after the 7th d after liver transplantation. Remaining models died of grade Ⅲ rejec-tion besides postoperative complication within 12 d after the transplantation. Conclusion The acute rejection of DA to LEW orthotopic liver transplantation models is stable and intensive. It can be effec-tively used to study allograft rejection and tolerance. But it is difficult to establish this kind of ortho-topic liver transplantation models in inbred rats because of their own tissue characteristics.