The nature of extra-chromosomal maintenance of transforming plasmids in the filamentous basidiomycete Phanerochaete chrysosporium |
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| Authors: | Thomas A. Randall C. Adinarayana Reddy |
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| Affiliation: | (1) Department of Microbiology, Michigan State University, 48824-1101 East Lansing, MI, USA;(2) Present address: Department of Physiological Chemistry, University of Wisconsin, 1300 University Avenue, 53705 Madison, WI, USA |
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| Abstract: | Summary The nature of extra-chromosomal maintenance of the transforming plasmid p12-6 in Phanerochaete chrysosporium was studied. Our results indicate that the plasmid is maintained in the fungal transformants extra-chromosomally as part of a larger endogenous plasmid (designated pME) of P. chrysosporium. Using the total DNA of p12-6 fungal transformants, p12-6, as well as a larger plasmid, p511, were recovered in recA–E. coli strains while only p12-6 was recovered in recA+E. coli strains. The results also showed that the cytosine methylation system has no apparent effect on the strain-dependent recovery of p12-6 and p511 in E. coli from the total DNA of fungal transformants. |
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| Keywords: | Phanerochaete chrysosporium Transformation Lignin degradation Filamentous fungi |
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