液相色谱-串联质谱法测定人血浆中盐酸舍曲林浓度

目的：建立测定人血浆中盐酸舍曲林浓度的液相色谱-串联质谱法。方法：以替米沙坦为内标，内标法定量。流动相：乙腈-10mmol·L^-1乙酸铵-1%甲酸（70：30：0.1）；质谱采用离子喷雾离子化源，扫描方式为多重反应监测（MRM），用于定量分析的离子反应分别为m/z306.3→m/z159.1（舍曲林）和m/z515.2→m/z276.1（替米沙坦）。结果：舍曲林和替米沙坦的保留时间分别为2.22min和2.44min；舍曲林的线性范围为0.5～50.0ng·mL^-1，r=0.9992，回归方程：Y=0.0021＋0.0217X，最低检测浓度为0.5ng·mL^-1；提取回收率在82%～90%范围内；日内相对标准差〈4%，日间精密度〈13%。结论：此法适合人体血浆盐酸舍曲林浓度的监测及生物利用度研究，结果准确、可靠。

Determination of Sertraline Hydrochloride in Healthy Volunteer Plasma by LC/MS/MS

Objective： To establish the LC/MS/MS method for the determination of sertraline hydrochloride. Methods： Telmisartan was taken as an internal standard. The assay was performed by Q-trap^TM liquid chromatography tandem mass spectrometric （MS/ MS） system with an electrospray ionization （ESI） using the multiple reaction monitoring. The ion monitoring mode at m/z 306.3 →m/z 159.1 for sertraline and m/z 515.2→m/z 276.1 for telmisartan were selected. Results： The retention time of sertraline and telmisartan were 2.22 min and 2.44 min, respectively. The linear response for sertraline was oh-rained in the range of 0.5 - 50.0 ng · mL^-1 with a correlation coefficient of 0.999 2. The limit of quantification was 0.5 ng · mL^-1. Precisions of intraday and interday assay were less than 4% and 13.0% respectively and the exaction recoveries were between 82% and 90%. Conclusion： The results indicate that the assay method has adequate precision and accuracy to determine the concentra-tion of sertraliue and its bioavailability in human plasma.