| 冠心病同型半胱氨酸与其相关酶基因MTHFR、CBS多态性关系的研究 |
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| 引用本文： | 方显明,唐耀平,郑德俊.冠心病同型半胱氨酸与其相关酶基因MTHFR、CBS多态性关系的研究[J].陕西医学杂志,2005,34(12):1457-1459,1463. |
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| 作者姓名： | 方显明 唐耀平 郑德俊 |
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| 作者单位： | 广西中医学院附属瑞康医院,南宁,530011 |
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| 基金项目： | 广西自然科学基金资助项目（No.桂科自0229046 |
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| 摘 要： | 目的:探讨冠心病血浆同型半胱氨酸(Hcy)水平与其代谢相关酶MTHFR、CBS基因型的关系。方法:采用试剂盒法提取人基因组DNA;采用聚合酶链反应-限制性片段长多态性(PCR-RFLP)法检测MTHFR基因型,采用扩增阻滞实变体系法(ARMS)检测CBS基因型。结果:冠心病患者MTHFR基因型异常者Hcy水平明显高于MTHFR基因型正常者,有显著差异(P<0.05);在本研究中没有检测出CBS基因突变者。结论:MTHFR基因突变是引起血浆Hcy水平升高的主要原因。
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| 关 键 词： | 冠状动脉疾病/病理学 半胱氨酸/血液 @亚甲基四氢叶酸还原酶 @胱硫醚-β-合成酶 基因 |
| 收稿时间： | 2005-05-08 |
| 修稿时间： | 2005-05-08 |
Study on homocysteine and MTHFR ,CBS gene polymorphism in patients with coronary heart disease |
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| Fang Xianming,Tang Yaoping,Zhen Dejun.Study on homocysteine and MTHFR ,CBS gene polymorphism in patients with coronary heart disease[J].Shaanxi Medical Journal,2005,34(12):1457-1459,1463. |
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| Authors: | Fang Xianming Tang Yaoping Zhen Dejun |
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| Institution: | RuiKang Hospital Attached to Guangxi College of Traditional Chinese Medicine, Nanning 530011 |
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| Abstract: | Objective : To explore relation between the level of plasma homocysteine(Hcy) and its relative enzyme gene MTHFR and CBS in patients with coronary heart disease. Methods:Human DNA was picked up by method of reagent box.The MTHFR genotypes were tested by the restriction fragment length polymorphism method(PCR-RFLP).The CBS genotypes were tested by the amplification refractory mutation system (ARMS)method. Result: The results show that the level of plasma Hcy is markedly higher in patients with gene mutation than in patients without gene mutation(P<0.05);the CBS genetic mutation is not found in this experiment.Conclusion: MTHFR genotype is one of main factors which cause level of Hcy to rise. |
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| Keywords: | Coronary disease/pathology Cysteine/blood @MTHFR @CBS Genes |
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