藏红花提取液对慢性高眼压兔视网膜SOD和MDA的影响

目的:探讨实验性慢性高眼压兔视网膜SOD和MDA含量的变化及藏红花提取液的保护作用。方法:新西兰家兔24只,随机分为正常对照组、高眼压组、治疗I组和治疗II组,每组6只12眼。高眼压组和2个治疗组兔的眼前房内注射3g/L复方卡波姆溶液0.2mL,造成慢性高眼压模型。治疗I,II组兔每日经耳缘静脉推注藏红花提取液,20mg和80mg原药/kg。在7,14d后处死各组半数动物,检测视网膜SOD,MDA含量,并进行组间比较分析。结果:造模7d后高眼压组、治疗I组和II组家兔视网膜组织中的SOD值均明显低于对照组,有显著统计学差异(P<0.05);但治疗I,II组的SOD值均明显高于高眼压组,且治疗II组高于治疗I组(P<0.05)。在14d时,高眼压组与2个治疗组的SOD值均已接近对照组水平,差异无显著性(P>0.05)。造模后7d,高眼压组视网膜组织中的MDA含量明显高于对照组,有显著统计学差异(P<0.01)。治疗I组、II组的MDA含量均低于高眼压组(P<0.01),但仍明显高于对照组(P<0.01)。造模后14d,高眼压组、治疗I组和II组的MDA水平均进一步升高,但与对照组相比仍略高(P<0.01)。结论:藏红花提取液在高眼压早期能有效提高慢性高眼压下视网膜中SOD活性,降低MDA含量。

Effect of crocus sativus on SOD and MDA alterations in the retina of rabbits with chronic ocular hypertension

·AIM:To observe the change of superoxide dismutase(SOD)and malondiadehyde(MDA)in the retina of rabbits with chronic ocular hypertension(OHT)and the protective effect of crocus sativus..METHODS:Twenty-four rabbits were randomly divided into four groups:control,OHT,and two crocus sativus-treated groups(n=6,totally 12 eyes for each group).Chronic ocular hypertension in OHT group and two crocus sativus-treated groups was induced by injecting 0.2mL of 3g/L Carbomer solution into anterior chamber.The rabbits in two treated groups were treated with extract preparation of crocus sativus every day,20mg/kg and 80mg/kg respectively.The animals were sacrificed at 7 or 14 days after Carbomer injection and the contents of superoxide dismutase(SOD)and malondiadehyde(MDA)in retina were assayed and compared among groups..RESULTS:The level of SOD in retinal tissue in OHT and treated groups were markedly lower than that in the control group on day 7 after modeling(P<0.05).The SOD values of two treated groups were obviously higher than that in OHT group and SOD level in the treated group with 80mg/kg crocus sativus was higher than that in the group treated with 20mg/kg crocus sativus(P<0.05).The level of MDA in retinal tissue in OHT and treated groups were markedly higher than that in the control group at day 7 after modeling(P<0.01).The MDA values of two treated groups were lower than that in OHT group(P<0.01)but still higher than the control(P<0.01).Fourteen days after modelling,the level of SOD in OHT group and two treated groups was comparable or near to that in control group,whereas MDA level was further increased and higher than that in control group(P<0.01)..CONCLUSION:Crocus sativus can effectively enhance the activity of SOD and reduce the content of MDA in retina in early period of OHT.Crocus sativus is an excellent antioxidant which can protect retina against early damage of free radicals..