| γ刀照射后胶质瘤细胞抑癌基因p16表达的变化 |
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| 引用本文： | 赵兴利,赵丛海,田宇,刘红强.γ刀照射后胶质瘤细胞抑癌基因p16表达的变化[J].吉林大学学报(医学版),2002,28(4):347-348,F003. |
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| 作者姓名： | 赵兴利 赵丛海 田宇 刘红强 |
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| 作者单位： | 1. 吉林大学中日联谊医院神经外科,吉林,长春,130031;2. 中国医学科学院医药生物技术研究所 |
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| 基金项目： | 卫生部科研项目;98-1-205; |
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| 摘 要： | 目的:观察γ刀对体外培养的胶质瘤C6细胞照射后,凋亡细胞中抑癌基因产物P16蛋白表达水平的变化.方法:体外培养胶质瘤C6细胞,经Leksell γ刀照射,体外培养48 h,应用免疫组化S-P法及计算机图像分析检测γ刀照射前后C6细胞P16蛋白表达水平.结果:5.00、6.22 Gy组γ刀照射后,培养48 h的C6细胞P16蛋白表达水平明显升高(细胞灰度值分别为155.4±2.0、124.9±7.1),与对照组(细胞灰度值为167.1±6.2)比较,差异有显著性(P<0.01).结论:γ刀诱导C6细胞凋亡的机理可能与激活P16蛋白表达水平有关.
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| 关 键 词： | γ刀 胶质瘤 p16基因 |
| 文章编号： | 1671-587X(2002)04-0347-02 |
The change of p16 gene expression in glioma cell line C6 after radiation with gamma knife |
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| ZHAO Xing li ,ZHAO Cong hai ,TIAN Yu ,LIU Hong qing.The change of p16 gene expression in glioma cell line C6 after radiation with gamma knife[J].Journal of Jilin University: Med Ed,2002,28(4):347-348,F003. |
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| Authors: | ZHAO Xing li ZHAO Cong hai TIAN Yu LIU Hong qing |
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| Institution: | ZHAO Xing li 1,ZHAO Cong hai 1,TIAN Yu 1,LIU Hong qing 2 |
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| Abstract: | Objective: To observe the change of expression of p16 gene prodact, P16 protein, after treated by gamma (γ)knife on glioma cell line C6. Methods: Glioma C6 cells proliferated in vitro, treated by γ knife in dose of 5 00 and 6 22 Gy,respectively. P16 protein was detected by immunohistochemical technique and immage analysis. Results: The P16 protein in glioma C6 cells was notably increased after treatment with γ knife ( P <0 01). The grey number in C6 group (control group) was 167 1±6 2 and was 155 4±2 0 and 124 9±7 1,respectively, in 5.00 Gy and 6.22 Gy gamma knife treated group. Conclusion: It is suggests that one of the mechanisms of glioma cell C6 apoptosis induced by γ knife radiation may be associated with activation of p16 gene and increase of P16 protein expression. |
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| Keywords: | knife glioma p16 gene |
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