雌二醇对内皮损伤后血管重塑的影响

目的 :观察雌二醇对血管内皮损伤后血管重塑的作用及可能机理。方法 :将雌性家兔行卵巢切除术后随机分为A、B、C组 ,A组每日注射 17 β雌二醇 40 0 μg/kg ,B组注射等量生理盐水 ,C组不注射。注射雌二醇 1周时3组均行右侧髂动脉近端内皮剥脱术 ,远端保持血管内皮完整 ,术后测量髂动脉管腔面积 (LA) ,内膜面积 (IA)、内弹力板环绕面积 (IEMA)、外弹力板环绕面积 (EEMA)。测定血管壁内皮剥脱处胶原纤维含量及分析Ⅰ /Ⅲ胶原比。结果 :A组较B、C组LA、IEMA、EEMA显著增大 ,负性血管重塑率显著减小。A组内皮剥脱处胶原含量较B、C组显著减少 ,三组之间Ⅰ /Ⅲ胶原比无显著差异。结论 :雌二醇能够改善血管重塑 ,减轻内皮损伤后的血管狭窄 ,这种良性血管重塑作用可能与雌激素可减少血管壁胶原含量 ,改善细胞外间质 (ECM)的代谢有关。

The Effect of Estradiol on Vascular Remodeling after Balloon injury

To evaluate estrogen's effect on vascular remodeling.Methods:A fter ovariectomy,all female rabbits were devided into 3 groups.Group A was inje cted 17-β estradiol 400 μg/(kg*d) subcutaneously.Group B with saline solution ,and group C with nothing.After one week,each rabbit was in process of right ili ac a rtery balloon injury.After one month of the procedure,Lumal area(LA),internal el astic membrane area(IEMA),external elastic membrane area (EEMA) were measured th rough computer analysis.Collagen and Ⅰ/Ⅲ collagen in the wall of the injured a r tery were evaluated by biochemical method.Results:The LA,IEMA and EEMA increased in group A significantly compared with those in the group B and C.Collagen in g roup A decreased significantly compared with group B and C.No difference of Ⅰ/ Ⅲ collagen was found within the three groups.Conclusion:Estradiol has a favorab le effect on vascular remodeling which contributes to ameliorating stenosis.And th is effect may be due to changes in collagen of wall of injured artery.