肝细胞癌中耐药基因的表达及意义

目的通过对肝细胞癌（HCC）中3种耐药基因MDR1、MRP、GST-π的检测,探讨肝细胞癌中耐药基因的表达特点及临床意义。方法应用逆转录-聚合酶链反应（RT-PCR）技术检测3种耐药基因在51例肝细胞癌组织和10例正常肝组织中的表达。结果 （1）MDR1、MRP、GST-π在肝细胞癌中的表达分别为0.55±0.27、0.62±0.29、0.64±0.31,正常肝组织中的表达分别为0.23±0.10、0.25±0.07、0.26±0.12,耐药基因在肝细胞癌中的表达高于正常肝组织,差异具有统计学意义（P〈0.05）;（2）耐药基因的表达与肿瘤Edmondson分级呈正相关（P〈0.05）;（3）MRP与GST-π的表达相关。结论肝细胞癌中存在有原发性耐药的现象,且多种机制并存。MDR1、MRP、GST-π在肝细胞癌中有较高的表达。联合检测对制定科学有效的治疗方案有一定价值。

The expression and clinical significance of resistance gene in hepatocellular carcinoma

Objective To detect the expression of three multidrug resistance genes（MDR1、MRP、GST-π） and evaluate clinical significances of multidrug resistance gene in hepatocellular carcinoma（HCC）.Methods RT-PCR was used to examine the expression of 3 resistance genes in 51 HCC cases and 10 normal tissues.Results The expression of MDR1、MRP、GST-π were 0.55±0.27,0.62±0.29,0.64±0.31 in hepatocellular cancer tissue and 0.23±0.10,0.25±0.07,0.26±0.12 in normal hepatic-tissue（P0.05）.The expressions of MDR1、MRP、GST-π in HCC were higher in hepatocellular carcinoma tissue than those in normal tissue.The expression of multidrug resistance genes were positively related to tumor Edmondson grade（P0.05）.The expression of MRP were correlated with the expression of GST-π.Conclusion Primary resistance existed in HCC and there were many mechanisms of multidrug resistance.MDR1,MRP,GST-π in hepatocellular carcinoma had a higher expression.Combined detection of LRP and GST-π expression maybe efficient in clinical treatment.