土三七水煎剂所致肝小静脉闭塞病大鼠模型的建立

目的通过观察不同浓度土三七水煎剂所致的大鼠肝脏病理学变化，建立一种模拟临床患者服药所致肝小静脉闭塞病（HVOD）的动物模型。方法土三七2100g水煎制成浓度为1．5g/ml土三七生药煎剂，SD大鼠76只随机分为A、B、C、D四组，A、B、C组每组20只，分别以土三七水煎剂高（15g·kg^-1·d^-1）、中（7．5g·kg^-1·d^-1）、低（3．75g·kg^-1·d^-1）浓度灌胃，D组16只以温开水灌胃作为对照，各组大鼠雌、雄各半。每日测量大鼠体重并记录异常表现，灌胃1周和2周后各组大鼠分别处死各半，肝脏标本行病理检查，HE及Masson染色，光镜下参照Deleve评分标准判定病变及严重程度。结果土三七高、中、低浓度组分别有83．3％（15／18）、75．0％（15／20）、40．0％（5／20）出现了肝小静脉闭塞病病理表现；相同剂量下雌性较雄性更易发病，雌、雄鼠造模成功率分别为79．3％（23／29）和41．3％（12／29）（P=0．003）。结论土三七水煎剂灌胃可以成功建立HVOD大鼠模型，剂量和大鼠性别与造模成功率直接相关。

A rat model of hepatic veno-occlusive disease induced by the Gynura root water decoction

Objective To study the pathological lesions of rat liver induced by the Gynura root water decoction, and establish a rat model for hepatic veno - occlusive disease （HVOD）. Methods 2100 gram Gynura root was prepared into the water decoction with the concentration of 1.5 g/ml. Group A, B and C with 20 SD rats were perfused intragastrically with the Gynura root water decoction with different concentrations of 15 g·kg^-1·d^-1 , 7.5 g·kg^-1·d^-1 and 3.75 g·kg^-1·d^-1, respectively. Group D was perfused intragastrically with water which served as the controls. The ratio of male to female was 1 ： 1 in every group. The body weights and symptoms were observed every day. Half of the rats from each group were killed and their liver samples were stained with H＆E and Masson techniques after one week and two weeks of drug administration respectively. Results The pathological manifestations of HVOD were observed in the 15 rats of 18 （83.3%） in group A, 15 of 20 （75.0%） in group B and 5 of 20 （40.0%） in group C. With the same dose, the female rats were prone to HVOD. After re - grouping by gender, pathological manifestations of HVOD could be found in 23 of the 29 male rats and 12 of the 29 female rats （79.3% vs 41.3%, P = 0. 003）. Conclusion The rat HVOD model could be induced by the Gynura root water decoction successfully which was associated with the concentration and gender.