A modified alkaline phosphatase enzyme amplification system and its application in an HIV antigen ELISA. |
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| Authors: | J K Ball G M Beards U Desselberger |
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| Affiliation: | Regional Virus Laboratory, East Birmingham Hospital, U.K. |
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| Abstract: | Alkaline phosphatase amplification systems increase the sensitivity of enzyme-linked immunoassays (ELISAs). Here we describe a modified method by which levamisole is a component of the substrate buffer. This increases sensitivity by reducing the signal from non-specific alkaline phosphatases. The modified procedure was applied to an 'in house' HIV-1 p24 antigen capture assay and was shown to increase the sensitivity 4-fold as compared to the unmodified system. The performance of the modified amplification system is comparable to that of commercially available systems. |
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