| 胚胎大鼠脊髓运动神经元体外培养方法的优化 |
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| 引用本文： | 宋学琴,吴淑玉,王丽琴,王晓绢,李春岩. 胚胎大鼠脊髓运动神经元体外培养方法的优化[J]. 河北医科大学学报, 2004, 25(3): 133-135,141 |
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| 作者姓名： | 宋学琴 吴淑玉 王丽琴 王晓绢 李春岩 |
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| 作者单位： | 河北医科大学第二医院神经内科,河北,石家庄,050000;河北医科大学第二医院神经内科,河北,石家庄,050000;河北医科大学第二医院神经内科,河北,石家庄,050000;河北医科大学第二医院神经内科,河北,石家庄,050000;河北医科大学第二医院神经内科,河北,石家庄,050000 |
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| 摘 要： | 目的建立一种胚胎大鼠脊髓运动神经元的体外分离培养方法。方法采用原代培养的方法，从孕15天胚胎大鼠的脊髓腹侧组织中分离脊髓前角神经元，通过差速贴壁法纯化培养细胞，应用神经元特异性的烯醇化酶抗体和抗神经微丝抗体-SMI32单克隆抗体对培养细胞进行鉴定。结果纯化培养体系中神经元占培养细胞的90％以上，其中70％左右为脊髓运动神经元(spina lmotor neurons，SMNS)。结论通过差速贴壁法纯化分离培养的SMNS体外生长状态良好，纯度高，可作为神经科学领域的重要研究手段。
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| 关 键 词： | 脊髓 运动神经元 细胞培养 胚胎 大鼠 体外研究 |
| 文章编号： | 1007-3205(2004)03-0133-03 |
OPTIMAL CONDITION OF CULTURED SPINAL MOTOR NEURONS IN VITRO FROM EMBRYONIC RAT |
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| SONG Xue-qin,WU Shu-yu,WANG Li-qin,WANG Xiao-juan,LI Chun-yan. OPTIMAL CONDITION OF CULTURED SPINAL MOTOR NEURONS IN VITRO FROM EMBRYONIC RAT[J]. Journal of Hebei Medical University, 2004, 25(3): 133-135,141 |
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| Authors: | SONG Xue-qin WU Shu-yu WANG Li-qin WANG Xiao-juan LI Chun-yan |
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| Abstract: | ObjectiveTo establish a culture method for spinal motor neurons (SMNs) from embryonic rat. MethodsThe SMNs were dissociated from embryonic 15 day rats and cultured in vitro. The culture system for SMNs was established using differential adhesion method for purification. The SMNs were identified by immunohistochemistry using neuron-specific enolase and SMI-32 mAb. ResultsMore than 90 % cells were neurons in this culture system and about 70 % or so were SMNs. ConclusionThe culture system established has a high purificating rate of SMNs and can provide a simple tool for the research in the field of neurology. |
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| Keywords: | spinal cord motor neurons cell culture embryo rats in vitro |
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