| 何首乌组织培养的研究 |
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| 引用本文： | 杜勤 符红. 何首乌组织培养的研究[J]. 中药材, 1998, 21(3): 109-110 |
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| 作者姓名： | 杜勤 符红 |
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| 作者单位： | 广州中医药大学 510407(杜勤，符红，詹若挺，姚幼玲)，广州中医药大学 510407(李雄威) |
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| 摘 要： | 采用单因子、正交设计法考察了基本培养基、激素以及光暗条件对何首乌幼嫩茎、叶诱导愈伤组织的影响,以及培养时间与愈伤组织生长量间的关系。实验研究表明:MS 2,4D1mg/L 6-BA1mg/L IBA0.5mg/L和暗培养对诱导何首乌愈伤组织产生效果较好;接种12天时愈伤组织生长量达到最大;愈伤组织经诱导能够产生完整植株,成苗率为95％。
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| 关 键 词： | 何首乌 组织培养 愈伤组织 诱导 植株 |
Study on Tissue Culture of Poligonum multiflorum |
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| Du Qin,Fu Hong,Than Ruoting,Yao Youling,Li Xongwei. Study on Tissue Culture of Poligonum multiflorum[J]. Journal of Chinese medicinal materials, 1998, 21(3): 109-110 |
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| Authors: | Du Qin Fu Hong Than Ruoting Yao Youling Li Xongwei |
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| Affiliation: | Guangzhou University of TCM, Guangzhou 510407. |
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| Abstract: | In the present thesis, suitable medium for callus inducement and relationship between culture time and callus growth of Poligonum multiflorum were studied by mono-factorial and orthogonal design test.The experiments showed that the best medium for callus inducement was MS 2,4 -D lmg/L 6-BA lmg/L IBA 0.5 mg/L, dark culture was better than light culture, callus grew fast when it was cultured 12 days, whole plant could be induced by callus, the induce rate was 95%. |
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| Keywords: | Poligonum multiflorum Tissue culture Callus Inducement Plant |
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