接受骨髓单个核细胞移植慢性肾损伤大鼠肾脏转化生长因子β1和结缔组织生长因子的表达

背景:近年来干细胞移植在肾脏病领域取得了一定进展,但多局限于急性肾损伤,涉及慢性肾损伤方面的报道较少,且肾脏保护机制不清楚.目的:观察同种异体骨髓单个核细胞移植后慢性肾损伤大鼠尿蛋白排泄、肾功能的变化,以及对.肾组织转化生长因子β1和结缔组织生长因子表达的影响.设计、时间及地点:随机对照动物实验,于2008-05/10在解放军沈阳军区总医院动物实验中心完成.材料:健康SPF级雌性SD大鼠30只,随机分为3组:正常对照组、模型组、细胞移植组,10只/组.另取雄性SD大鼠10只用于骨髓单个核细胞的制备.方法:模型组、细胞移植组大鼠采用改良肾脏5/6切除术建立慢性肾损伤模型,造模成功后10周,细胞移植组大鼠心脏内注射骨髓单个核细胞悬液1.0 mL,细胞数1×10~8个,只,治疗4周;模型组心脏内注射等量生理盐水.主要观察指标:24 h尿蛋白定量及肾功能变化,肾组织病理学变化,肾组织转化生长因子β1及结缔组织生长因子蛋白的表达.结果:①与正常对照组比较,模型组、细胞移植组大鼠尿蛋白排泄量及血清尿素、肌酐浓度均显著升高(P

Effect of bone marrow mononuclear cells transplantation on the expression of transforming growth factor beta 1 and connective tissue growth factor in chronic renal damage rats

BACKGROUND: The stem cell transplantation achieved progress in treating acute kidney injury. However, cell transplantation in treating chronic renal damage, as well as the protection mechanism, remains poorly understood.OBJECTIVE: To observe the changes of urine protein and renal function, as well as the effects of bone marrow mononuclear cells on expression of transforming growth factor β1 (TGF-β1) and connective tissue growth factor (CTGF) in chronic renal damage rats.DESIGN, TIME AND SETTING: The randomized controlled animal experiment was performed at the Experimental Animal Center of General Hospital of Shenyang Military Command from May to October 2008.MATERIALS: Totally 30 female SD rats, with SPF grade, were randomly divided into the control, model and cell transplantation groups, with 10 animals in each group. Additional 10 SD rats were used to prepare bone marrow mononuclear cells.METHODS: Chronic renal damage models were prepared by modified hepatic resection, and 1.0 mL mononuclear cells were injected at 10 weeks after model preparation, with 1×10~8 cells per animal. Rats in the model group were injected same volume of physiological saline.MAIN OUTCOME MEASURES: The 24 hour urinary protein excretion quantity and renal function changes, histopathological change of renal tissues, as well as the expression of TGF-β1 and CTGF.protein excretion, urea nitrogen and creatinine levels (P < 0.01), which especially greater in the model group (P < 0.01 or P