山羊腰椎间盘退变模型硬膜外胶原酶的注射*

背景：采用椎间盘外、硬膜外腔注射胶原酶治疗椎间盘突出症的机制，目前尚不完全清楚。 目的：观察硬膜外注射胶原酶对椎间盘退变动物模型的作用。 方法：成年山羊6只，麻醉后作侧外方切口至腰椎体腹侧，椎板加压并用钢板螺钉固定，L1/L2，L3/L4椎间盘分别注射0.5 mL无水乙醇建立椎间盘退变模型。硬膜外注射实验用胶原酶1 mL，2周后处死动物，取出椎间盘，作电镜切片观察。 结果与结论：电镜下显示：退变的椎间盘纤维环上有裂隙，退变的椎间盘的胶原纤维明显溶解，未退变的椎间盘未有明显溶解。提示硬膜外注射胶原酶通过纤维环上的裂隙渗透到盘内，发生化学溶解作用，从而达到治疗作用。

Epidural injection of collagenase into a goat model of lumbar disc degeneration

BACKGROUND: The mechanism of epidural injection of collagenase to treat lumbar disc degeneration is still unclear. OBJECTIVE: To assess the mechanism of epidural injection of collagenase in animal models of lumbar disc degeneration. METHODS: Six adult sheep were excessively pressed with steel plate and L1/L2, L3/L4 segments were injected with 0.5 mL absolute alcohol to prepare lumbar disc degeneration models. Two weeks after epidural injection of collagenase, the sheep was killed to take the disc for electronic microscope observation. RESULTS AND CONCLUSION: The electronic microscope showed that there were some fissures in the fibrous ring of the degenerated intervertebral disk, collagen fibers discs were dissolved, non-degenerative discs were unchanged. Collagenase can enter degenerated intervertebral disc and directly dissolve nucleus pulposus tissue.