| PEDF肽段44-mer对前列腺癌细胞PC-3分化作用的研究 |
| |
| 引用本文： | 孙耀飞,方祖军,关明,郑捷,童仕俊,孙传玉.PEDF肽段44-mer对前列腺癌细胞PC-3分化作用的研究[J].上海医学检验杂志,2010(12). |
| |
| 作者姓名： | 孙耀飞 方祖军 关明 郑捷 童仕俊 孙传玉 |
| |
| 作者单位： | 复旦大学附属华山医院泌尿外科;复旦大学附属华山医院中心实验室; |
| |
| 基金项目： | 上海市自然科学基金项目(08ZR1402200) |
| |
| 摘 要： | 目的观察色素上皮细胞衍生因子(PEDF)肽段44-mer(57-101)诱导激素非依赖性前列腺癌(PCa)细胞PC-3发生神经内分泌分化(NED),并探讨其对前列腺癌的治疗机理。方法体外合成44-mer并对PC-3的生长进行干预,以采用不同浓度44-mer诱导作为实验组,未用44-mer诱导作为对照组。从形态、标志物两方面证实部分细胞发生NED;采用水溶性四氮唑(WST-1)、流式细胞仪检测诱导后细胞的增殖情况及细胞周期。建立荷瘤裸鼠模型,实验组腹腔注射44-mer,定期测量肿瘤体积,并对标本进行免疫组化染色以检测嗜铬粒素A(ChrA)的表达情况;对照组腹腔注射磷酸盐缓冲液(PBS),并定期测量肿瘤体积。结果实验组PC-3中发生NED形态学改变的细胞所占的比率为18.65%±1.26%,明显高于对照组(3.62%±0.44%)(P<0.01);1、10、100 nmol/L44-mer分别诱导PC-3 3 d后培养液中的ChrA水平分别为(168.97±5.36)、(263.95±5.30)、(325.24±5.74)ng/mL,均高于对照组(115.97±2.16)ng/mL](P均<0.01);免疫印迹...
|
| 关 键 词： | 前列腺癌 神经内分泌分化 色素上皮细胞衍生因子 肽段 |
Study on the effect of PEDF peptide 44-mer on differentiation of prostate cancer cell line PC-3 |
| |
| SUN Yaofei,FANG Zujun,GUAN Ming,ZHENG Jie,TONG Shijun,SUN Chuanyu..Study on the effect of PEDF peptide 44-mer on differentiation of prostate cancer cell line PC-3[J].Shanghai Journal of Medical Laboratory Sciences,2010(12). |
| |
| Authors: | SUN Yaofei FANG Zujun GUAN Ming ZHENG Jie TONG Shijun SUN Chuanyu |
| |
| Institution: | SUN Yaofei1,FANG Zujun1,GUAN Ming2,ZHENG Jie1,TONG Shijun1,SUN Chuanyu1.(1.Department of Urology,Huashan Hospital,Fudan University,Shanghai 200040,China,2.Central Laboratory,Shanghai,200040,China) |
| |
| Abstract: | Objective To observe the neuroendocrine differentiation(NED) effect of pigment epithelium-derived factor(PEDF) peptide 44-mer(57-101) on the androgen independent prostate cancer(PCa) cell line PC-3 and investigate the therapeutic mechanism of this peptide for PCa.Methods PC-3 were treated with 44-mer which were synthesized in vitro.The subjects induced by different concentrations of 44-mer were perfromed as experiment group,and they treated without 44-mer were performed as controls.NED was confirmed by morp... |
| |
| Keywords: | Prostate cancer Neuroendocrine differentiation Pigment epithelium-derived factor Peptide |
| 本文献已被 CNKI 等数据库收录！ |
|
