|
|||||
|
|
| 河南省腹泻病人5种致泻大肠埃希菌感染调查 | |
| 引用本文： | 朱敏,谢志强,赵嘉咏,邓文斌,张锦,郭万申,王子军,冉陆.河南省腹泻病人5种致泻大肠埃希菌感染调查[J].现代预防医学,2010,37(6). |
| 作者姓名： | 朱敏 谢志强 赵嘉咏 邓文斌 张锦 郭万申 王子军 冉陆 |
| 作者单位： | 1. 中国生物技术发展中心,北京,100036 2. 河南省疾病预防控制中心 3. 中国疾病预防控制中心 |
| 摘 要： | 目的]应用多重PCR技术筛查自腹泻病人中分离的致泻大肠埃希菌,了解5种致泻大肠埃希菌的感染情况。方法]用常规方法分离培养粪便标本中的大肠埃希菌,提取DNA,采用多重PCR扩增12对目的基因,电泳检测扩增产物,确定病原检出情况,并进行统计学分析。结果]2007年河南省1534份标本中致泻大肠埃希菌检出率10.37%,其中EAEC(3.59%)居首位,其余依次检出EPEC(2.67%)、STEC(2.15%)、ETEC(1.63%)、EIEC(0.33%)。合并感染检出率0.78%,以ETEC合并感染居多。感染高峰在5月(19.85%),城市与农村的检出率差异无统计学意义,45～60岁组腹泻病人的检出率最高(17.00%)。结论]应用多重PCR技术能快速鉴别粪便标本中的致泻大肠埃希菌。致泻大肠埃希菌是河南省常见的腹泻病原。 |
| 关 键 词： | 多重PCR 致泻大肠埃希菌 腹泻 |
RESEARCH ON THE DETECTION OF FIVE TYPES OF DIARRHEAGENIC ESCHIERICHIA COLI FROM DIRRHEAL PATIENTS IN HENAN PROVINCE |
|
| ZHU Min,XIE Zhi-qiang,ZHAO Jia-yong,et al..RESEARCH ON THE DETECTION OF FIVE TYPES OF DIARRHEAGENIC ESCHIERICHIA COLI FROM DIRRHEAL PATIENTS IN HENAN PROVINCE[J].Modern Preventive Medicine,2010,37(6). | |
| Authors: | ZHU Min XIE Zhi-qiang ZHAO Jia-yong |
| Institution: | ZHU Min,XIE Zhi-qiang,ZHAO Jia-yong,et al. (China National Center for Biotechnology Development,Beijing 100036,China) |
| Abstract: | Objective] To screen five types of diarrheagenic Escherichia coli isolated from diarrheal patients with multiplex PCR technique and to clarify the detected rate of pathogens. Methods] Escherichia coli strains were isolated from stool specimens and cultured according to the conventional method. Then DNA was extracted. Twelve templates were amplified with multiplex PCR technique. The products of amplification were analyzed by electrophoresis. The diarrheal patients with pathogenic infections were epidemiolo... |
| Keywords: | Multiplex PCR Diarrheagenic escherichia coli Diarrhea |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |