胶质瘤细胞血小板源生长因子B链的纯合二聚体与其受体基因？…

目的 探讨胶质瘤细胞血小板源生长因子Ｂ链的纯合二聚合（ＰＤＧＦＢＢ）及其受体（ＤＧＦＲ）基因表达和ＰＤＧＦＲ活化汪洋在胶质瘤发生、发展听作用。方法 用原位杂交和免疫组化染色观察了７３例不同级别的人胶质瘤组织标本。结果 ６２例（８４．９％）的肿瘤细胞表达ＰＤＧＦＢｍＲＮＡ,基阳性率和阳性肿瘤细胞含量均随肿瘤恶性程度升高而递增。ＰＤＧＦＲα、ＰＤＧＦＲβ和这两种受体及其信号传递通路活化标记物酪氨酸磷酸

A study of gene expression of PDGFBB and PDGFR as well as level of PDGFR activity in human glioma cells

OBJECTIVE: To investigate the effect of PDGFBB autocrine loop on the development and progression of human gliomas. METHODS: 73 human glioma specimens of different stages were studied using in situ hybridization and immunohistochemical techniques. RESULTS: 62 (84.9%) of 73 cases expressed PDGFB mRNA. The positive rate and the amount of PDGFB mRNA increased correspondingly with the degree of malignancy. All of 73 gliomas expressed PDGFR alpha, PDGFR beta and phosphotyrosine protein (P-Tyr). The positive cell densities of PDGFR alpha, PDGFR beta and P-Tyr correlated positively with one another and increased with the malignancy as well as the expression level of PDGFB mRNA in the gliomas. However, there was no significant difference between positive cell densities of PDGFR alpha and PDGFR beta in any glioma group with different grading. CONCLUSIONS: PDGFBB autocrine loop existed generally in the glioma cells, the expression levels of PDGFBB and PDGFRs were well correlated with the tumor grading and the positive cell density of P-Tyr which could objectively reflect the activity of PDGFRs and the signal pathways. It's suggested that abnormally increased activity of PDGFBB autocrine loop might play an important role in the development and progression of gliomas.