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CNTF对大鼠脊髓损伤后逆行性红核神经元损伤的保护作用
引用本文:叶俊丽,曹莉,崔瑞耀,黄爱军,阎志勇,路长林,何成.CNTF对大鼠脊髓损伤后逆行性红核神经元损伤的保护作用[J].第二军医大学学报,2002,23(6):598-601,F004.
作者姓名:叶俊丽  曹莉  崔瑞耀  黄爱军  阎志勇  路长林  何成
作者单位:1. 第二军医大学基础医学部神经生物学教研室,上海,200433青岛大学青岛医学院病理生理学教研室,青岛,266021
2. 第二军医大学基础医学部神经生物学教研室,上海,200433
3. 青岛大学青岛医学院病理生理学教研室,青岛,266021
基金项目:上海市现代生物与新药产业发展基金 (0 0 43 192 0 3 ),国家 973重大科研基金资助项目 (G19990 5 40 0 0 )
摘    要:目的:研究睫状神经营养因子(CNTF)对脊髓损伤后逆行性红核神经元损伤的保护作用和促进肢体功能恢复的作用。方法:将大鼠分为假手术组,生理盐水(NS)组和CNTF组,用Allen改良法撞击致SD大鼠脊髓T10损伤,经蛛网膜下腔留置导管注射NS和CNTF;术后每周进行一次BBB评分和斜板试验的行为学评分;6周时采用辣根过氧化物酶(HRP)逆行示踪技术标记红核神经元,取红部位脑组织作冰冻切片,应用四甲基联苯胺(TMB)呈色反应显示脊髓损伤后红核神经元存活率,并进行图像数据的分析。结果:(1)CNTF组行为功能高于NS组;(2)CNTF组HRP标记红核神经元数目多于对照组。结论:CNTF可减轻脊髓损伤后的逆行性损伤,而对上行运行神经元发挥保护作用,从而对肢体功能的恢复起到促进作用。

关 键 词:CNTF  大鼠  脊髓损伤  逆行性红核神经元损伤  保护作用  睫状神经营养因子  HRP
文章编号:0258-879X(2002)06-0598-04

Neuroprotective effect of ciliary neurotrophic factor on rubrospinal neurons following spinal cord injury in adult rats
YE Jun Li ,CAO Li ,CUI Rui Yao ,HUANG Ai Jun ,YAN Zhi Yong ,LU Chang Lin ,HE Cheng.Neuroprotective effect of ciliary neurotrophic factor on rubrospinal neurons following spinal cord injury in adult rats[J].Academic Journal of Second Military Medical University,2002,23(6):598-601,F004.
Authors:YE Jun Li    CAO Li  CUI Rui Yao  HUANG Ai Jun  YAN Zhi Yong  LU Chang Lin  HE Cheng
Institution:YE Jun Li 1,2,CAO Li 1,CUI Rui Yao 2,HUANG Ai Jun 1,YAN Zhi Yong 1,LU Chang Lin 1,HE Cheng 1*
Abstract:Objective:To study the neuroprotective effects of ciliary neurotrophic factor(CNTF) on rubrospinal neurons and functional recovery in a contusion model of spinal cord injury (SCI) in the adult rat. Methods:SD rats were divided into 3 groups:sham operated group, NS and CNTF group. NS and CNTF were given through intrathecal catheter for 10 d after T 10 were injured by modified Allen contusion method. Animals were behaviorally tested for 6 weeks using the Basso, Beattie, Bresnahan locomotor rating scale and inclined plane test. At the end week 6, rubrospinal neurons of 5 rats in each group were labeled by retrograde transport of the horseradish peroxidase (HRP) from the lesion site, which were taken by damaged axons and remained in the neurons, then the labeled red nucleus neuron (RN) were counted. Results:(1) Rats treated with CNTF had more improvements in hindlimb function than controls. (2) The amount of survival rubrospinal neurons was significantly higher in CNTF treated animals than in controls. Conclusion:Intrathecal infusion of exogenous CNTF following SCI significantly reduces retrograde neuronal cell death and enhances functional recovery. CNTF plays a neuroprotective role against spinal cord injury in the adult rat.
Keywords:spinal cord injury  ciliary neurotrophic factor  red nucleus neuron  HRP
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