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| 细菌生物膜与导管伴生感染相关性的研究 | |
| 引用本文： | 刘新,王岚,刘颖,张晓科.细菌生物膜与导管伴生感染相关性的研究[J].沈阳医学院学报,2010,12(3):133-135. |
| 作者姓名： | 刘新 王岚 刘颖 张晓科 |
| 作者单位： | 1. 沈阳医学院基础医学院病原生物学研究室,辽宁省环境污染与微生态学重点实验室,辽宁沈阳110034 2. 解放军沈阳202医院干诊科 3. 中国医科大学附属第一医院重症监护病房 |
| 基金项目： | 辽宁省教育厅基金课题 |
| 摘 要： | 目的：探讨细菌生物膜在置入导管伴生感染中的作用。方法：对置入性导管于撤除导管时行导管内细菌检查及分离培养,用结晶紫和阿利新蓝染色法对细菌生物膜进行定位及定量检测,分析导管细菌生物膜形成与导管伴生感染的关系。结果：患者置入性导管细菌生物膜阳性率为61.29%（38/62）,染色技术可用于观察导管内细菌生物膜形成情况。结论：细菌生物膜形成是置入导管伴生感染的重要致病因素,染色技术简便快速,可作为临床快速诊断的方法。 |
| 关 键 词： | 生物膜 导管 伴生感染 |
The Correlation Research of Bacterial Biofilm with Associated Infection of Cathter |
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| LIU Xin,WANG Lan,LIU Ying,ZHANG Xiao-ke.The Correlation Research of Bacterial Biofilm with Associated Infection of Cathter[J].Journal of Shenyang Medical College,2010,12(3):133-135. | |
| Authors: | LIU Xin WANG Lan LIU Ying ZHANG Xiao-ke |
| Institution: | 1.Department of Pathogenic Bizlogy,Shenyang Medical College,Key Lab of Enviromental Pollution and Microecology of Liaoning Province,Shenyang 110034,China;2.Department of Cadres Diagnosis and Treatment,People Liberation Army 202 Hospital;3.ICU,The First Hospital Affiliated to China Medical University) |
| Abstract: | Objective： To investigate the effect of bacterial biofilm on associated infection of insertion catheter.Methods：The bacteria in insertion catheter were isolated and cultured after removing catheter.The method of staining with Crystal Violet and Alcian Blue was used to localization and quantitation detection of bacterial biofilm.Then the relationship of bacterial biofilm with associated infection of catheter was analyzed.Results：The positive ratio of bacterial biofilm in insertion catheter was 61.29%（38/62）.The staining method can be used to observe the formation condition of bacterial biofilm in insertion catheter.Conclusion：The formation of bacterial biofilm was important pathogenic factor in associated infection of insertion catheter.The staining technique was convenient and fast that can be used in clinical fast diagnosis. |
| Keywords: | biofilm catheter associated infection |
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