恶性黑素瘤的端粒酶活性研究

目的检测恶性黑素瘤标本中的端粒酶活性,探讨端粒酶在黑素瘤发生机制中的作用。方法采用聚合酶链反应-酶联免疫吸附测定法(PCR-ELISA),对黑素瘤标本进行端粒酶的定性和定量分析。结果90.0％的原发性黑素瘤和100％的转移性黑素瘤显示端粒酶阳性,仅14.3％的黑素细胞痣呈阳性,而正常皮肤中均为阴性。转移性黑素瘤、原发性黑素瘤、黑素细胞痣和正常皮肤的端粒酶活性水平的平均A值分别为0.729、0.405、0.118和0.044。各组端粒酶活性A值之间经统计学分析差异有显著性。结论大部分恶性黑素瘤患者中存在高水平的端粒酶活性,提示端粒酶可能在黑素瘤的发生机制中起重要作用,对黑素瘤的诊断有一定的临床意义。

Study of Telomerase Activity in the Patients with Malignant Melanoma

Objective To detect telomerase activity in the patients with melanoma and study the role of telomerase in the tumorigenesis of melanoma.Methods The technique of polymerase chain reaction- enzyme linked immunosorbent assay (PCR-ELISA) was employed to detect telomerase activity in the patients with melanoma. Results 90. 0% primary melanoma and 100% metastatic melanoma had positive telomerase activity. Only 14.3% melanocytic nevus showed positive telomerase activity. But the normal skins had no telomerase activity. A value of telomerase activity was 0. 729,0.405,0.118 and 0.044 in metastatic melanoma,primary melanoma,melanocytic nevus and normal skin,respectively. Differences of telomerase activity were statistically significant between these groups. Conclusion The most of patients with malignant melanoma had high level of telomerase activity, suggesting that telomerase might play an important role in the tumorigenesis of melanoma and become a useful marker for the diagnosis of melanoma.