丙型肝炎基因型定量检测及分型检测方法的研究进展

丙型肝炎病毒(hepatitis C virus,HCV)是一种RNA病毒,可以引发各种慢性肝病,其中包括肝硬化和肝细胞癌.全球每年HCV感染的患者数呈上升趋势,他已成为人类亟待解决的公共卫生难题.HCV可以分为6种主要的基因型以及70多种亚型,不同的基因型对抗病毒治疗的效果不同.如果在治疗前能通过准确灵敏的检测手段确定HCV的基因型,将会对临床治疗有重要的意义.本文对HCV基因型全球分布、临床表症与治疗、分型依据以及基因型与定量关系进行了概述,并重点阐述HCV基因分型的检测技术.

Research progress in hepatitis C virus quantity and genotyping assays

Hepatitis C virus is an RNA virus that can cause liver disease,including cirrhosis and hepatocellular carcinoma.Since cases of HCV infection have been increasing annually,HCV infection has become a global health problem. Six different genotypes of HCV and more than 70 subtypes have been identified,and patients infected with different HCV genotypes show different responses to antiviral therapy. Therefore,determination of the HCV genotype by accurate and sensitive methods will have a significant impact on clinical therapy.This review summarizes the information about hepatitis C genotyping and global distribution, its clinical symptoms and treatment methods, genotype evidence,the relationship between the genotype and quantity,and genotyping assays.