右旋松萝酸对小鼠口服急性毒性及细胞毒性的实验研究

目的 观察右旋松萝酸的口服急性毒性,MTT方法测其细胞抑制活性.方法 建立小鼠经口急性中毒小鼠模型,观察不同剂量药物灌胃小鼠的中毒表现.测定小鼠的经口LD_(50),观察不同剂量右旋松萝酸对小鼠生存时间及存活率的影响.同时,用不同浓度的右旋松萝酸作用于体外培养的心肌成纤维细胞,MTT方法测定细胞的IC_(50),观察不同浓度药物对细胞的抑制率.结果 右旋松萝酸剂量越大,小鼠的中毒症状越明显,生存时间越短.小鼠经口LD-(50)为388mg/kg,中毒表现主要有精神萎靡,耸毛,畏寒,呼吸急促,行动迟缓,厌食等症状.体外细胞培养实验显示,右旋松萝酸对细胞增值的抑制呈剂量正相关,较大剂量对细胞有破坏作用,其对心肌成纤维细胞的IC_(50)为322 μg/ml.结论 右旋松萝酸为一种中低毒的天然化合物,中低剂量没有明显的细胞毒性.

Abstract：

Objective To observe the oral acute toxicity of of (+)-usnic acid in mice and assess its cytotoxicity in rat cardiac fibroblasts. Methods The mice with acute poisoning of (+)-usnic acid at different doses by oral administration were observed for toxic manifestations, and the LD_(50) was determined. The survival time and survival rate of the mice receiving different doses of (+)-usnic acid were observed. Cultured rat cardiac fibroblasts were inoculated with different concentrations of (+)-usnic acid, and the cell growth inhibition rate was estimated and the IC_(50), determined using MTT assay. Results Higher dose of (+) -usnic acid resulted in more obvious symptoms of poisoning and shorter survival time of the mice. The LD_(50) of (+)-usnic acid in mice by oral administration was 388 mg/kg. The manifestations of poisoning such as apathism, pilomotor, chill, dyspnea,torpidity and anorexia was observed. Rat cardiac fibroblasts incubated with (+)-usnic acid showed obvious growth inhibition,which was positively correlated to the dose of (+)-usnic acid, and high dose of (+)-usnic acid caused severe cell injuries. The IC_(50) of (+)-usnic acid in rat cardiac fibroblasts was 322 μg/ml. Conclusion (+)-usnic acid is a natural compound of low toxicity in mice, and low to medium dose of (+)-usnic acid dose not produce obvious cytotoxicity.

Oral acute toxicity of (+)-usnic acid in mice and its cytotoxicity in rat cardiac fibroblasts

Objective To observe the oral acute toxicity of of (+)-usnic acid in mice and assess its cytotoxicity in rat cardiac fibroblasts. Methods The mice with acute poisoning of (+)-usnic acid at different doses by oral administration were observed for toxic manifestations, and the LD50 was determined. The survival time and survival rate of the mice receiving different doses of (+)-usnic acid were observed. Cultured rat cardiac fibroblasts were inoculated with different concentrations of (+)-usnic acid, and the ce...