改良式液氮冷冻法制备犬股骨头坏死动物模型

目的 通过改良式液氮冷冻法造成犬股骨头缺血坏死,探讨股骨头缺血坏死的动物模型制备,建立能模拟人股骨头坏死病理生理过程的实验动物模型.方法 16只犬随机分成4组,采用手术方法改良式液氮冷冻法即刻冷冻单侧股骨头.普通饲料饲养2、4、8、16周分别处死4组动物,分别观察X线摄片、大体形态、光镜下形态变化.结果 改良式液氮冷冻法犬股骨头X线、大体及组织学形态不同阶段均出现明显变化.结论 改良式液氮冷冻法为建立股骨头坏死动物模型提供了一种简便、易行的方法,值得进一步深入研究.

Abstract：

Objective To establish an canine model of femoral head osteonecrosis using an improved liquid nitrogen freezing method. Methods Sixteen adult canines were divided into 4 groups at random and subjected to instant freezing of the unilateral femoral head with liquid nitrogen. The dogs were observed at 2, 4, 8, and 16 weeks after the operation for radiographic, gross and pathological changes of the femoral head. Results Significant radiographic, gross and pathological changes occurred in the femoral head after the freezing. Conclusion Improved liquid nitrogen freezing of the femoral head provides a simple and convenient method for establishing animal models of femoral head osteonecrosis.

An improved liquid nitrogen freezing method for establishing canine models of femoral head necrosis

Objective To establish an canine model of femoral head osteonecrosis using an improved liquid nitrogen freezing method. Methods Sixteen adult canines were divided into 4 groups at random and subjected to instant freezing of the unilateral femoral head with liquid nitrogen. The dogs were observed at 2, 4, 8, and 16 weeks after the operation for radiographic, gross and pathological changes of the femoral head. Results Significant radiographic, gross and pathological changes occurred in the femoral head after ...