表没食子儿茶素没食子酸酯对人大肠癌株SW620增殖的抑制及与PAK1基因表达的相关性研究

目的 观测表没食子儿茶素没食子酸酯(epigallocatechin-3-gallate,EGCG)对人大肠癌SW620细胞增殖及SW620细胞表达PAK1的影响.方法 用终浓度为40 μmol/L、60 μmol/L和80 ixmo1/L的EGCG干预人大肠癌细胞株SW620,在0、24、48和72 h不同时间点上用噻唑蓝(MTT)比色法检测EGCG对SW620细胞生长的影响;并以SW620细胞空白组为对照,用蛋白免疫印迹(Westerm blot)检测不同EGCG作用48 h时SW620细胞PAK1的表达水平变化.结果 EGCG对大肠癌SW620细胞增殖有显著的抑制作用,而且与SW620细胞空白对照组相比.EGCG处理组细胞的PAK1蛋白表达受到显著抑制.结论 EGCG抑制大肠癌SW620细胞增殖的机制可能与EGCG通过调控大肠癌细胞的PAK1表达相关.

Abstract：

Objective To investigate the effects of epigallocatechin-3-gallate (EGCG) on the proliferation of SW620 cells and the expression of PAK1 gene. Methods Human colonic cancer cell line SW620 was treated with EGCG at 40, 60 and 80 μmol/L and cultured in RPMI 1640 medium for 0, 24, 48 and 72 h. The proliferation of SW620 cells was observed by MTT assay before and after EGCG treatment, and the expression of PAK1 protein was observed by Western blotting. Results SW620 cells treated with EGCG displayed a slowed growth in comparison with the control cells, and the growth rate decreased with the increase of EGCG concentration. PAK1 protein expression was lowered in SW62Q, cells after EGCG treatment for 48 h. Conclusion EGCG can inhibit the proliferation and partially reduce the expression of PAK1 protein in SW620 cells.

Effects of epigallocatechin-3-gallate on the proliferation of colonic cancer cell line SW620 and PAK1gene expression

Objective To investigate the effects of epigallocatechin-3-gallate (EGCG) on the proliferation of SW620 cells and the expression of PAK1 gene. Methods Human colonic cancer cell line SW620 was treated with EGCG at 40, 60 and 80 μmol/L and cultured in RPMI 1640 medium for 0, 24, 48 and 72 h. The proliferation of SW620 cells was observed by MTT assay before and after EGCG treatment, and the expression of PAK1 protein was observed by Western blotting. Results SW620 cells treated with EGCG displayed a slowed growth in comparison with the control cells, and the growth rate decreased with the increase of EGCG concentration. PAK1 protein expression was lowered in SW62Q, cells after EGCG treatment for 48 h. Conclusion EGCG can inhibit the proliferation and partially reduce the expression of PAK1 protein in SW620 cells.