| TRIM28对肺鳞癌SK-MES-1细胞生长的影响 |
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| 引用本文： | 马春虎,许倩,沈荣飞,肖铄洋,李冰清,任祎. TRIM28对肺鳞癌SK-MES-1细胞生长的影响[J]. 承德医学院学报, 2015, 0(1): 5-8 |
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| 作者姓名： | 马春虎 许倩 沈荣飞 肖铄洋 李冰清 任祎 |
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| 作者单位： | 1. 承德医学院,河北承德,067000;2. 承德医学院基础医学院2012级临本10班 |
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| 基金项目： | 河北省自然科学基金项目，河北省教育厅资助项目 |
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| 摘 要： | 目的：：探讨TRIM28基因对肺鳞癌细胞系SK-MES-1细胞生长的影响。方法：采用RNA干扰技术抑制肺鳞癌SK-MES-1细胞TRIM28基因的表达，通过RT-PCR和Western-blot技术检测抑制效果，应用克隆形成实验和MTT实验观察TRIM28基因被抑制后SK-MES-1细胞的增殖和活性。结果：RNA干扰技术能有效抑制TRIM28基因在肺鳞癌SK-MES-1细胞中的表达。TRIM28基因被抑制后，肺鳞癌SK-MES-1细胞的活性和生长受了到明显抑制。结论：TRIM28对肺鳞癌SK-MES-1细胞的生长具有促进作用。
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| 关 键 词： | 肺鳞癌 TRIM28 RNA干扰 |
INFLUENCES OF TRIM28 ON GROWTH OF LUNG SQUAMOUS CELL CARCINOMA SK-MES-1 CELLS |
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| Affiliation: | MA Chunhu;XU Qian;SHEN Rong-fei;Chengde Medical College; |
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| Abstract: | Objective:To study the influences of TRIM28 gene on growth of lung squamous cell carcinoma SKMES- 1 cells.Methods:TRIM28 gene was inhibited by RNA interference assay, RT-PCR and Western-blot was used to detect the inhibitory effects. After the TRIM28 gene was inhibited, the proliferation and activity of SK-MES-1 cells were detected respectively by clone formation assay and MTT cell proliferation assay.Results:Expression of TRIM28 in SK-MES-1 cells was inhibited significantly by RNA interference. The activity and growth of SK-MES-1 cells decreased obviously after the TRIM28 gene was inhibited.Conclusions:TRIM28 can promote the growth of lung squamous cell carcinoma SK-MES-1 cells. |
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| Keywords: | Lung squamous cell carcinoma TRIM28 RNA interference |
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