P16和PCNA在骨肉瘤中的表达及临床意义

目的：研究P16和增殖细胞核抗原（PCNA）在骨肉瘤中的表达及其与临床病理参数的关系，探讨两者在骨肉瘤发生、发展中的作用。方法：应用免疫组织化学方法（SP）对10例正常骨组织和71例骨肉瘤组织中的P16与PCNA蛋白进行定量检测，并结合临床资料进行分析。结果：①P16在骨肉瘤组织中表达的阳性率明显低于正常骨组织（P<0.05），PCNA在骨肉瘤组织中表达的阳性率明显高于正常骨组织（P<0.05）；②P16表达与骨肉瘤的组织学分级、预后有关（P<0.05）,与组织学类型、临床分期无关（P>0.05）。PCNA表达与骨肉瘤的预后有关（P<0.05），与临床分期、组织学分级及组织学类型无关（P>0.05）。③P16与PCNA表达呈负相关关系(r_s=－0.58，P<0.05)。结论：P16及PNCA在骨肉瘤的发生、发展和转移过程中起着重要作用，与骨肉瘤的预后有关。

Expressions of P16 and proliferating cell nuclear antigen in osteosarcoma and clinical significances

Objective To investigate the relationship between the expressions of P16 and proliferating cell nuclear antigen(PCNA) in osteosarcoma and clinicopathological features,and explore the effects of them in occurrence and development of osteosarcoma.Methods The expressions of P16 and PCNA were detected by immunohistochemistry(SP) in 71 osteosarcima tissues and 10 normal bone tissues.Results ①The positive rate of P16 expression in osteosarcoma tissues was lower than that in normal bone tissues(P<0.05),the positive rate of PCNA expression in osteosarcoma tissues was higher that in normal bone tissues(P<0.05).②The expression of P16 was correlated with osteosarcima's histological grade and prognosis(P<0.05),and wasn't correalted with histological type and clinical stage(P>0.05).The expression of PCNA had positive relationship with osteosarcoma's prognosis(P<0.05),and had no relationship with histological type and clinical stage and histological grade(P>0.05).③There was negative correlation between P16 and PCNA expressions(rs=-0.58,P<0.05).Conclusion P16 and PCNA can play an important role in the occurreace,development and the metastasis of osteosarcoma,and have close relationship with its prognosis.