半枝莲提取物诱导人肺癌SPC-A-1细胞凋亡及对凋亡相关基因表达的影响

目的:探讨半枝莲提取物(Scutellaria barbataextract,SBE)对人肺癌SPC-A-1细胞凋亡及凋亡相关基因survivin和caspase-3表达的影响。方法:体外培养肺癌SPC-A-1细胞,以2.5、5、10 mg/L SBE处理SPC-A-1细胞48h,并以2.5 mg/L顺铂(DDP)作为阳性对照。用MTT法检测细胞抑制率,TUNEL法检测凋亡率,半定量RT-PCR检测survivin和caspase-3 mRNA表达。结果:与正常对照组相比,SBE各组细胞抑制率显著上升(P<0.001),细胞凋亡率明显升高(P<0.01),Caspase-3 mRNA表达显著上升(P<0.05或P<0.01),Survivin mRNA表达明显下降(P<0.05或P<0.01)。结论:SBE具有诱导肺癌SPC-A-1细胞凋亡的作用,其分子机制可能与上调caspase-3及下调survivin基因表达有关。

Apoptosis-inducing Effect of Scutellaria barbata Extract on Human Lung Cancer SPC-A-1 Cells and the Expression of Apoptosis Associated Genes

Objective: To explore the apoptosis-inducing effect of Scutellaria barbata extract(SBE) and the expression of apoptosis associated genes survivin and caspase-3 on human lung cancer SPC-A-1 cells.Methods: Lung cancer SPC-A1 cells were treated with 2.5,5 and 10 mg/L for 48 h,and the cells were treated with 2.5 mg/L DDP as positive control.The inhibitory rat was evaluated by MTT assay.Apoptotic rate was determined by TUNEL method.The expression of survivin and caspase-3 mRNA were detected by semi-quantitive RT-PCR.Results: Compared with control group,the inhibitory rate was increased obviously(P<0.001),the apoptotic rate was increased markedly(P<0.01),the expression of caspase-3 mRNA was increased significantly(P<0.05 or P<0.01),while survivin mRNA was decreased markedly(P<0.05 or P<0.01) in SBE groups.Conclusion: SBE can induce apoptosis of SPC-A-1 cells.The molecular mechanism may be related to up-regulating expression of caspase-3 and down-regulating expression of survivin genes.