| SPGL诱导HL-60细胞分化作用的研究 |
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| 引用本文： | 杨华中,张建辉,宋奎,孙小娟. SPGL诱导HL-60细胞分化作用的研究[J]. 中药材, 2007, 30(10): 1273-1275 |
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| 作者姓名： | 杨华中 张建辉 宋奎 孙小娟 |
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| 作者单位： | 1. 湖南师范大学医学院,湖南长沙,410006
2. 中南大学湘雅医学院,湖南长沙,410007 |
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| 摘 要： | 目的:研究光叶海桐总皂苷(SPGL)诱导HL-60细胞分化的作用。方法:采用1×10-5~5×10-4mg/ml浓度SPGL处理第3 d的HL-60细胞进行Wrigh-Giemsa染色、NBT还原能力及细胞表面抗原的检测。结果:在药物作用下,HL-60细胞形态学出现分化特征;NBT还原能力增强,且其阳性细胞数与SPGL的浓度有明显的量-效关系;CD11b与CD14表面抗原的表达明显升高。结论:SPGL有诱导HL-60细胞分化的作用,并诱导其向粒细胞系和单核系细胞分化。
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| 关 键 词： | HL-60细胞 分化 白血病 |
| 文章编号： | 1001-4454（2007）10-1273-03 |
| 修稿时间： | 2007-03-08 |
Study of Differentation of HL-60 Cells by Induction of SPGL |
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| YANG Hua-zhong,ZHANG Jian-hui,SONG kui,SUN Xiao-juan. Study of Differentation of HL-60 Cells by Induction of SPGL[J]. Journal of Chinese medicinal materials, 2007, 30(10): 1273-1275 |
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| Authors: | YANG Hua-zhong ZHANG Jian-hui SONG kui SUN Xiao-juan |
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| Affiliation: | 1. Medical College of Hunan Normal University, Hunan Changsha 410006,China; 2. Xiangya Medical College of Central South University, Hunan Changsha 410007, China |
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| Abstract: | OBJECTIVE: To study the effect of differentiation of HL-60 cells by induction of SPGL. METHODS: Cell differentiation was analyzed by using Wrigh-Giemsa staining to observe the morphology changes of cells with microscope, NBT reductant test, surface differentiation antigen (CD11b and CD14) test in HL-60 cells treated by different doses (1 x 10(-5) - 5 x 10(-4) ng/ml) of SPGL at the 3th day. RESULTS: The morphological changes showed cell differentiation characteristics; NBT reductant was significantly increased, and the number of NBT positive cells were related to dose of SPGL (in dose-dependent manner); Expression of CD11b and CD14 increased obviously. CONCLUSION: The results indicate that the effect of differentiation of HL-60 cells by induction of SPGL and it induces the cells to differentiate along the monocyte and granulocyte lineage. |
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| Keywords: | SPGL |
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