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SPGL诱导HL-60细胞分化作用的研究
引用本文:杨华中,张建辉,宋奎,孙小娟. SPGL诱导HL-60细胞分化作用的研究[J]. 中药材, 2007, 30(10): 1273-1275
作者姓名:杨华中  张建辉  宋奎  孙小娟
作者单位:1. 湖南师范大学医学院,湖南长沙,410006
2. 中南大学湘雅医学院,湖南长沙,410007
摘    要:目的:研究光叶海桐总皂苷(SPGL)诱导HL-60细胞分化的作用。方法:采用1×10-5~5×10-4mg/ml浓度SPGL处理第3 d的HL-60细胞进行Wrigh-Giemsa染色、NBT还原能力及细胞表面抗原的检测。结果:在药物作用下,HL-60细胞形态学出现分化特征;NBT还原能力增强,且其阳性细胞数与SPGL的浓度有明显的量-效关系;CD11b与CD14表面抗原的表达明显升高。结论:SPGL有诱导HL-60细胞分化的作用,并诱导其向粒细胞系和单核系细胞分化。

关 键 词:HL-60细胞  分化  白血病
文章编号:1001-4454(2007)10-1273-03
修稿时间:2007-03-08

Study of Differentation of HL-60 Cells by Induction of SPGL
YANG Hua-zhong,ZHANG Jian-hui,SONG kui,SUN Xiao-juan. Study of Differentation of HL-60 Cells by Induction of SPGL[J]. Journal of Chinese medicinal materials, 2007, 30(10): 1273-1275
Authors:YANG Hua-zhong  ZHANG Jian-hui  SONG kui  SUN Xiao-juan
Affiliation:1. Medical College of Hunan Normal University, Hunan Changsha 410006,China; 2. Xiangya Medical College of Central South University, Hunan Changsha 410007, China
Abstract:OBJECTIVE: To study the effect of differentiation of HL-60 cells by induction of SPGL. METHODS: Cell differentiation was analyzed by using Wrigh-Giemsa staining to observe the morphology changes of cells with microscope, NBT reductant test, surface differentiation antigen (CD11b and CD14) test in HL-60 cells treated by different doses (1 x 10(-5) - 5 x 10(-4) ng/ml) of SPGL at the 3th day. RESULTS: The morphological changes showed cell differentiation characteristics; NBT reductant was significantly increased, and the number of NBT positive cells were related to dose of SPGL (in dose-dependent manner); Expression of CD11b and CD14 increased obviously. CONCLUSION: The results indicate that the effect of differentiation of HL-60 cells by induction of SPGL and it induces the cells to differentiate along the monocyte and granulocyte lineage.
Keywords:SPGL
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