毛细管区带电泳测定尿17—羟皮质类固醇

目的：应用高效毛细管区带电泳（CZE）技术分离和检测人尿内17－羟类皮质类固醇（17－OHCS），以解决微色谱柱－分光光度测定尿17－OHCS时，多酚类化合物对测定的干扰，方法：标本用活性高岭土吸附尿内色素，以茶碱作内标，中性苯乙烯－二乙烯苯树脂微色谱柱吸附尿中17－OHCS，乙醇解吸附，采用毛细管区带电泳进一步分离并测定，结果：运行CZE法分离了人尿17－OHCS，皮质醇浓度在2－50mg/L^-1范围内具有良好的线性关系（r=0.994)，方法平均回收率96．17％，日内及日间CV均小于5％，最低检测限度为0．3mg.L^-1，结论：与微色谱柱－分光光度法相比，CZE法检测尿内17－OHCS无干扰，方法简便，快速，可靠。

Determination of 17-hydroxycorticosteroid by capillary zone electrophoresis]

OBJECTIVE: To detect and dissociate 17-hydroxycorticosteroids(17-OHCS) in urine by using high performance capillary zone electrophoresis(CZE) so as to solve the problem that phenolic compounds interfere with the determination of urine 17-OHCS by chromatographic-spectrophotometry. METHODS: Pigments were absorbed from samples by activated kaolin in which the internal standard was theophyline. Urine 17-OHCS was absorbed by neutral styrene-divinylbenzene resin. Thus pigments and phenolic compounds were separated roughly. Then 17-OHCS was washed down by alcohol and separated and detected by capillary zone electrophoresis. RESULTS: Urinary 17-OHCS was separated successfully. Urinary 17-OHCS had a good linearity in the range of 2-50 mg.L-1 (r = 0.994). The average recovery rate was 96.17%. The relative standard deviation was less than 5%. The consistence detection limit was 0.3 mg.L-1. CONCLUSIONS: Compared with chromatographic-spectrophotometry, the high performance capillary electrophoresis has much less interference and is more simple, rapid, and accurate.