人肝癌HepG2细胞超微弱发光的观察

目的：探讨人肝癌HepG2细胞系超微弱发光的特征。方法：用IFFM—D型流动式化学发光仪检测人肝细胞株QZG及人肝癌HepG2细胞系超微弱发光的强度以及不同浓度鲁米诺（Luminol）和双氧水（H2O2）对其超微弱发光的影响。结果：在10^-4mol／L鲁米诺及0．3％双氧水条件下，人肝癌HepG2细胞超微弱发光强度明显高于人肝细胞株QZG（P〈0．05），并随细胞浓度增高其超微弱发光强度呈线性增高，其差异显著（P〈0．05）；提高鲁米诺浓度为10^-3mol／L时，人肝癌HepG2细胞发光强度明显增加（P〈0．05）；提高双氧水浓度为3％时，其超微弱发光强度的变化无显著差异（P〉0．05）。结论：人肝癌HepG2细胞的超微弱发光强度明显高于人肝细胞株QZG,细胞浓度及鲁米诺、双氧水浓度变化能影响细胞超微弱发光强度。超微弱发光反映了肿瘤细胞的功能状态，检测肿瘤细胞超微弱发光强度可作为一项敏感的肿瘤细胞生理及代谢指标。

Observation of ultraweak luminescence of human hepatocarcinoma HepG2 cell

Objective:To explore the characteristics of the ultraweak luminescence of human hepatocarcinoma cells(HepG2). Methods: The intensity of the ultraweak luminescence of HepG2 cells and human hepatocytes(QZG)was examined by an IFFM-D chemiluminescence instrument.The intensity of the ultraweak luminescence of HepG2 cells and human hepatocytes(QZG)were affected by different concentration of Luminol and H_(2)O_(2). Results: In 10~(-4)mol/l Luminol and 0.3% H_(2)O_(2),the intensity of the ultraweak luminescence of HepG2 cells was significantly higher than that of QZG cells(P<0.05).With cell number increasing,the intensity of the ultraweak luminescence of HepG2 cells increased obviously(P<0.05).The concentration of Luminol increased to 10~(-3)mol/l,the intensity of the ultraweak luminescence of human HepG2 cells increased obviously(P<0.05).While the concentration of H_(2)O_(2) increased to 3%,the increasing of the intensity of the ultraweak luminescence of cells were not obvious((P>)0.05). Conclusion:The intensity of the ultraweak luminescence of HepG2 cells is higher than that of QZG cells.The intensity of the ultraweak luminescence is affected by cell number and the concentration of Luminol and H_(2)O_(2).The ultraweak luminescence reflect functional status of tumor cells.It can become an sensitive index of physiology and metabolism of tumor cells.