平阳霉素磁性微球的制备及特性检测

目的：制备平阳霉素磁性微球，对其特性进行检测并评价其靶向性。方法：采用冷冻凝聚法制备包裹超微Fe203和平阳霉素的明胶微球，测定微球的直径；应用液相高效色谱仪、原于吸收分光光度仪、药物溶出仪等到定微球各成分含量、释放率及在外加磁场作用下微球在大鼠体内的分布情况。结果：微球呈球形，平均直径32．23μm各成分含量为(W／W)：Fe30411．7％，PYM3．8％，明胶84．5％。3h内平阳霉素释放率63．1％。Fe3O4在大鼠靶向部位分布明显高于非靶向部位。结论：平阳霉素磁性微球具有较好的靶向性和缓释性，是治疗海绵状血管瘤较为理想的剂型。

Preparation and the characteristic test of magnetic pingyangmycine-bearing microspheres]

OBJECTIVES: To prepare the magnetic pingyangmycine-bearing microspheres (MPMs), test its characteristics, and evaluate the target ability of MPMs. METHODS: MPMs were prepared by a cold-condensation method at first. Then the scan electron microscope, high performance liquid chromatography (HPLC) and atomic absorption spectrometry were used to measure the diameter of MPMs and the contents of PYM and Fe3O4 in MPMs. Drug-releasing instrument was used to test the quantity of PYM released from MPMs. The distribution of intravenously injected MPMs in SD rat liver was investigated under an externally magnetic field. RESULTS: MPMs are spherical with the mean diameter of 32.23 microns. The content of pingyangmycine in MPMs was 3.8% and Fe3O4 was 11.7% (w/w). The quantity of PYM released from MPMs in 3 hours was 63.1%. The content of Fe3O4 in the targeting sites of rats was obviously higher than that in the non-targeting sites (P < 0.01). CONCLUSION: MPMs have good properties of drug-targeting and drug sustain releasing. MPMs may be ideal for the use of the treatment of cavernous hemangioma.