苦参碱对大鼠慢性环孢素肾毒性模型TGF-β1和BMP-7表达的影响

目的：探讨苦参碱对大鼠慢性CsA肾毒性是否具有保护作用及其可能机制.方法：SD大鼠56只,随机分为对照组（橄榄油0.2 ml/d）,CsA模型组（CsA 20 mg·kg^-1·d^-1）,安博维组（CsA 20 mg·kg^-1·d^-1＋安博维10 mg·kg^-1·d^-1）和苦参碱组（CsA 20 mg·kg^-1·d^-1＋苦参碱10 mg·kg^-1·d^-1）,实验共4周,于第2周和第4周末2次采集肾脏标本,行常规病理检查,免疫组化检测TGF-β1和BMP-7表达.结果：与对照组比较,CsA组小管间质损伤评分,TGF-β1表达明显增加,而BMP-7明显下降,P＜0.05.苦参碱能下调TGF-β1表达,促进BMP-7表达,减轻肾脏小管间质损伤,P＜0.05.结论：苦参碱对实验性大鼠慢性CsA肾毒性具有一定的保护作用.

The Effect of Matrine on the Expression of TGF - β1 and BMP - 7 in Experimental Chronic Cyclosporine Nephropathy in Rats

Objective： To investigate the renal protective effect and possible mechanism of matrine in experimental cyclosporine A - induced chronic nephrotoxicity, Methods： Fifty six male Sprague - Dawley rats were randomly divided into four groups：control group（olive oil 0.2 ml/d）, CsA groap（CsA 20 mg·kg^-1·d^-1）, Ab group（CsA 20 mg·kg^-1·d^-1 plus Ab 10 mg·kg^-1· d^- 1 ） ,matrine group（CsA 20 mg· kg^- 1·d^- 1 plus matrine 100 mg· kg^- 1 ·d^- 1 ）, with fourteen rats in each group. The rats were sacrificed at the end of weeks 2,4. The kidneys were resected and fixed in 10% neutral formaldehyde for HE staining. Transforming growth factor- beta I（TGF- β1）, bone morphogenic protein- 7 （BMP- 7） were determined by irnmunohistochemical method. Results：Compared with controt group, the histologic scores of the tubular- interstitial injury and the expression of TGF- β1 of CsA group were significantly increased, BMP- 7 was significantly lower,P〈0.05. Matrine can dowrtregulate TGF-β1 expression but upregulate BMP- 7 expression in kidney compared with CsA group, P〈 0, 05. Conclusion： Matrine has a renoprotective effect on chronic cyclost：ofine nephrotoxicity rat model.