镉中毒肝脏过氧化氢(H2O2)定位及抗氧化系统的变化

为探讨急性镉中毒后自由基产生的损伤的部位及机理，给予大鼠尾静脉注射氯化镉建立急性镉中毒模型，电镜观察自由基过氧化氢（Ｈ２Ｏ２）的定位及细胞损伤，并测定抗氧化酶和氧化剂的变化。结果表明：Ｈ２Ｏ２－ＣｅＣｌ３的阳性颗粒沉积在肝细胞膜上，最早出现在中毒后１ｈ，３ｈ最严重，２４ｈ消失；抗氧化酶活力受抑，以３ｈ最严重（Ｐ〈０．０１），６ｈ后渐渐恢复；而ＧＳＨ的含量在３ｈ前均高于正常（Ｐ〈０．０５），以后逐步

Study on Hydrogen Preoxide(H2O2) Localization and Antioxidative System Changes of Hepatocytes After Cadmium-exposure

To study the localization and mechanism of free radical production and injury by cadmium,we set up the toxical model by intravenous injecting cadmium chloride into rats.Then injury of hepatocytes and free radical(H 2O 2) localization were studied by electron microscopy,and contents of antioxidation were determinated.Results Showed H 2O 2 CeCl 3 positive precipitation on outer plasma membrane of hepatocytes first appeared at 1h after cadmium exposure,increased obviously at 3h and disappeared at 24h.Activities of antioxidase decreased clearly and with the serious in 3h(p<0 01),recovered progressively in 6h.But GSH content increased obviously before 3h(p<0 05),and decreased step by step.Conclusion:Free radical production is the important factor on injury of hepatocytes after cadmium exposure.