温心胶囊对大鼠实验性缺血心肌细胞凋亡及Bcl-2、Fas蛋白表达的影响

目的：探讨临床有效中药复方温心胶囊防治冠心病心肌缺血的分子作用机制。方法：采用盐酸异丙肾上腺素连续多点皮下注射制备大鼠心肌缺血模型。运用透射电镜技术及TUNEL法，观察温心胶囊对缺血损伤大鼠心肌细胞凋亡的影响，并运用免疫组化的方法观察其对细胞凋亡相关基因Bcl-2、Fas蛋白表达的影响。结果：异丙肾上腺素所致心肌缺血损伤可明显诱导大鼠心肌细胞发生凋亡，显著引发Fas基因蛋白表达增强，并轻度引发Bcl-2基因蛋白表达增强，温心胶囊可显著下调Fas基因的蛋白表达，上调Bcl-2基因的蛋白表达，明显抑制和阻断心肌细胞发生凋亡。结论：通过调节缺血心肌Bcl-2、Fas基因的蛋白表达从而抑制和阻断细胞凋亡的发生，可能是中药复方温心胶囊防治冠心病心肌缺血损伤的分子作用机制之一。

Effect of Wenxin Capsule on Myocyte Apoptosis and Expression of Bcl-2, Fas Gene Protein in Rats of Experimental Myocardial Ischemia

Objective: To explore the molecular mechanism of Wenxin Capsule (WXC, an effective Chinese composite drug) in preventing and treating myocardial ischemia of coronary heart disease. Methods: Rat model of myocardial ischemia was established by subcutaneous multi-point injecting isoproterenol. Effect of WXC on cell apoptosis was observed by transmission electron microscopy and TUNEL method, and its effect on apoptotic related gene Bcl-2 and Fas gene protein expression was observed by immunohistochemical method. Results: Isoproterenol induced myocardial ischemic injury could cause evident cardial cell apoptosis, obvious enhance Fas gene protein expression and mild enhance Bcl-2 gene protein expression. WXC could significantly down-regulate Fas, up-regulate Bcl-2 gene protein expression, significantly inhibit and block the myocardial cell apoptosis. Conclusion: To inhibit and block the event of cell apoptosis through regulating Bcl-2 and Fas gene protein expression in ischemic myocardium might be one of the mechanisms of WXT in preventing and treating myocardial ischemic injury of coronary heart disease