| HPLC法测定复方四嗪利血平片中氢氯噻嗪的含量 |
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| 引用本文： | 陈玉璞. HPLC法测定复方四嗪利血平片中氢氯噻嗪的含量[J]. 中国医药导报, 2010, 7(5): 55-56 |
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| 作者姓名： | 陈玉璞 |
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| 作者单位： | 焦作市食品药品检验所,河南焦作,454000 |
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| 摘 要： | 目的：建立高效液相法测定复方四嗪利血平片中氢氯噻嗪含量的方法。方法：采用Diamonsil-C18（6．0mm×150mm，5μm）色谱柱；流动相：甲醇-水-磷酸盐缓冲液（30：66：4）（0．05mol／L磷酸二氢钾溶液，用磷酸调节pH至3．0）；检测波长：272nm；流速：1．0ml／min。结果：氢氯噻嗪在0．0896-0．0896μg范围内与峰面积呈良好的线性关系（r=0．9999），平均回收率为98．92％，RSD=0．60％（n=6）。结论：本方法简便、准确、重现性好，可用于复方四嗪利血平片中氢氯噻嗪的质量控制。
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| 关 键 词： | 高效液相法 氢氯噻嗪 复方四嗪利血平片 含量测定 |
Content determination of hydrochlorothiazide in Compound Tatrazin and Reserpine Tablets by HPLC |
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| CHEN Yupu. Content determination of hydrochlorothiazide in Compound Tatrazin and Reserpine Tablets by HPLC[J]. China Medical Herald, 2010, 7(5): 55-56 |
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| Authors: | CHEN Yupu |
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| Affiliation: | CHEN Yupu (Institute for Food and Drug Control of Jiaozuo City, Jiaozuo 454000, China) |
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| Abstract: | Objective： To establish a method for the content determination of hydrochlorothiazide in Compound Tatrazin and Reserpine Tablets by HPLC. Methods： The Diamonsil-C18（6.0 mm×150 mm, 5 μm） column was used. The mobile phases consisted of methanol, water and phosphate buffer solution （3066：4） [adjust Potassium dihydrogen phosphate, Solution （0.05 mol/L） to pH=3.0 by phosphoric acid], the flow rate was 1.0 ml/min and the detection wavelength was at 272 nm. Results： The linear ranges of hydrochlorothiazide was at 0.089 6-0.089 6 μg （r=0.999 9）, the average recovery was 98.92% with a RSD of 0.60% （n=6）. Conclusion： The method is fast, reliable, accurate and can be used in the quality control of hydrochlorothiazide in Compound Tatrazin and Reserpine Tablets. |
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| Keywords: | HPLC Hydrochlorothiazide Compound Tatrazin and Reserpine Tablets Content determination |
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