| Labeling of human insulin-like growth factor-Ⅰ eukaryotic expression vector with green fluorescent protein |
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| 引用本文： | 刘一,张绍昆,吴宏,单玉兴,王刚,徐莘香.Labeling of human insulin-like growth factor-Ⅰ eukaryotic expression vector with green fluorescent protein[J].中华创伤杂志(英文版),2005,8(3):132-137. |
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| 作者姓名： | 刘一 张绍昆 吴宏 单玉兴 王刚 徐莘香 |
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| 作者单位： | [1]DepartmentofOrthopaedicSurgery,FirstHospital,JilinUniversity,Changchun130021,China [2]DepartmentofOphthamology,SecondUniversity,Changchun130041,China |
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| 摘 要： | To label human insulin-like growth factor-Ⅰ (hIGF-Ⅰ) eukaryotic expression vector with green fluorescent protein (GFP) for the repair of articular cartilage defects. Methods: GFP cDNA was inserted into pcDNA3.1-hIGF-Ⅰ to construct the co-expression vector with two multiple cloning sites mammalian expression vector under two cytomegalovirus promoters/enhancers respectively. Recombinant pcGI was transfected into NIH 3T3 cells with the help of lipofectamine. Results : Enzyme digestion and agarose gel electrophoresis analysis revealed that pcGI vector contained correct GFP and hIGF-Ⅰ eDNA. Expression of hIGF-Ⅰ and GFP was confirmed in transfected NIH 3T3 cells by immunocytochemical analysis and fluorescence microscopy. Conclusions : hIGF-Ⅰ eukaryotic expression vector has been successfully labeled with GFP.
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| 关 键 词： | 胰岛素样生长因子-Ⅰ 真核细胞 绿荧光蛋白质 关节软骨损伤 |
Labeling of human insulin-like growth factor-I eukaryotic expression vector with green fluorescent protein. |
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| Yi Liu,Shao-kun Zhang,Hong Wu,Yu-xing Shan,Gang Wang,Xin-xiang Xu.Labeling of human insulin-like growth factor-I eukaryotic expression vector with green fluorescent protein.[J].Chinese Journal of Traumatology(English Edition),2005,8(3):132-137. |
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| Authors: | Yi Liu Shao-kun Zhang Hong Wu Yu-xing Shan Gang Wang Xin-xiang Xu |
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| Institution: | Department of Orthopaedic Surgery, First Hospital, Jilin University, Changchun 130021, China. |
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| Abstract: | OBJECTIVE: To label human insulin-like growth factor-I (hIGF-I) eukaryotic expression vector with green fluorescent protein (GFP) for the repair of articular cartilage defects. METHODS: GFP cDNA was inserted into pcDNA(3.1)-hIGF-1 to construct the co-expression vector with two multiple cloning sites mammalian expression vector under two cytomegalovirus promoters/enhancers respectively. Recombinant pcGI was transfected into NIH 3T3 cells with the help of lipofectamine. RESULTS: Enzyme digestion and agarose gel electrophoresis analysis revealed that pcGI vector contained correct GFP and hIGF-I cDNA. Expression of hIGF-1 and GFP was confirmed in transfected NIH 3T3 cells by immunocytochemical analysis and fluorescence microscopy. CONCLUSIONS: hIGF-I eukaryotic expression vector has been successfully labeled with GFP. |
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