大鼠周围突向躯体和内脏分支投射的脊神经节细胞内一氧化氮和降钙素基因相关肽共存

目的 :寻找电刺激肋间神经调节胃功能活动的化学神经基础。材料和方法 :用荧光素双标记结合还原型尼克酰胺腺苷二核苷酸磷酸 -黄递酶 ( nicotin amide adenine dinucleotide phosphate- diaphorase,NADPH- d)组化方法和降钙素基因相关肽 ( calcitonin gene- related peptide,CGRP)免疫组化方法 ,观察了大鼠周围突向肋间神经和胃分支投射的脊神经节细胞是否含一氧化氮 ( nitric oxide,NO)或 NO和 CGRP。结果 :分别有 2 1.6%和 11.8%的周围突向肋间神经和胃分支投射的脊神经节细胞呈 NADPH- d阳性和 NADPH- d/ CGRP阳性 ,4 7.9%的胃初级传入脊神经节细胞呈 NADPH- d/ CGRP阳性。结论 :大鼠周围突向躯体和内脏分支投射脊神经节细胞内含 NO或 NO和CGRP共存 ,NO和 CGRP还共存于内脏初级传入脊神经节细胞。

COEXISTENCE OF NITRIC OXIDE AND CALCITONIN GENE RELATED PEPTIDE IN SPINAL GANGLIA CELLS WITH DICHOTOMIZING PERIPHERAL PROCESSES TO BOTH SOMATO VISCERAL STRUCTURES IN RATS

Objective:In order to investigate the chemical nerve basis for regulating the stomach functions by electric stimulation to the intercostal nerves. Methods: Retrograde fluorescent double tracing combining with nicotinamide adenine dinucleotide phosphate diaphorase(NADPH d) histochemical and CGRP immunohistochemical method were used to investigate whether the spinal ganglia cells with dichotomizing peripheral processes to the intercostal nerves and stomach contain nitric oxide(NO) or NO and calcitonin gene related peptide(CGRP). Results: 21.6% and 11.8% of the spinal ganglia cells with dichotomizing peripheral processes to both the intercostal nerves and stomach were NADPH d and NADPH d/CGRP positive respectively, and 47.9% of the stomach afferent spinal ganglia cells were NADPH d/CGRP positive. Conclusion: NO exists or colocalizes with CGRP in the spinal ganglia cells with dichotomizing peripheral processes to both the somato visceral structures, NO and CGRP are also co located in visceral afferent spinal ganglia cells in rats.