脑瘫患儿脑脊液氨基酸类神经递质的变化

目的：研究痉挛型脑瘫患儿肌痉挛形成的机制。方法：采用丹酰氯-聚酰胺薄膜层析法，测定45例痉挛型脑瘫患儿和30例非神经系统疾病儿童脑脊液中兴奋性及抑制性氨基酸水平。结果：脑瘫组脑脊液中γ-氨基丁酸明显低于对照组，谷氨酸、天冬氨酸明显高于对照组，甘氨酸与对照组比较差异无显著性；谷氨酸的水平与患者肌张力呈等级正相关(rs=0.642, P<0.05)。 结论：兴奋性突触功能上升，突触前抑制和突触后抑制下降，脊神经后根传入侧支出芽突触重建可能在痉挛型脑瘫肌痉挛的形成中起重要作用。

Change of amino acid neurotransmitter in cerebrospinal fluid in children with cerebral palsy

Objective To investigate the mechanism of spasticity in cerebral palsy(CP). Methods Cerebrospinal fluid was obtained from spastic cerebral palsy group (CP, n =45), control group (children without central nervous system disenses, n =30). The concentrations of inhibitory amino acids (IAAs) and excitatory amino acids (EAAs) in cerebrospinal fluid were determined by dimethylamino naphthalene s sulfonyl chloride with polyamine thin layer chromatography. Results The level of GABA was significantly lower than that of the control group ( P <0 001) whereas GA and Asp were significantly higher than that of the control group ( P <0 001). The level of GA correlated positively with muscular tone. The level of Gly was not significantly different from that of the control group.Conclusion The increase of excitatory postsynaptic potential (EPSP),the decrease of presynaptic inhibition and postsynaptic inhibition and the formation of new synapses by sprouting of afferent axon terminals may play an important role in spasticity of cerebral palsy.