| 白及多糖对糖尿病溃疡创面愈合的作用研究 |
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| 引用本文： | 俞林花,聂绪强,潘会君,凌霜,章丹丹,卞卡.白及多糖对糖尿病溃疡创面愈合的作用研究[J].中国中药杂志,2011,36(11):1487-1491. |
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| 作者姓名： | 俞林花 聂绪强 潘会君 凌霜 章丹丹 卞卡 |
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| 作者单位： | 1. 上海中医药大学穆拉德中药现代化研究中心,上海,201203
2. 上海中医药大学穆拉德中药现代化研究中心,上海201203;上海教委一氧化氮和炎症医学E-研究院,上海201203 |
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| 基金项目： | 国家"重大新药创制"科技重大专项(2009ZX09311-003);上海市教委高校一氧化氮与炎症医学E研究院计划项目(E-04010);上海市科委基础研究重点项目(08DZ1972104) |
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| 摘 要： | 目的:研究白及多糖对糖尿病溃疡愈合的治疗作用及其作用机制.方法:SD大鼠56只,糖尿病模型通过腹腔注射链脲佐菌素(STZ,50 mg·kg-1)建立,72 h后,血糖大于≥16.7 mmol·L-1即认为糖尿病造模成功.4周后,切割2个直径为1.8 cm大小圆的全层皮肤,糖尿病溃疡模型制造后,2个创面分别用白及多糖和对照PBS干预;于伤后3,7,14,21 d时相点对创面愈合面积、组织形态学改变、成纤维细胞(FB)增殖情况、羟脯氨酸(OHP)含量、髓过氧化物酶(MPO)含量进行观察与检测.结果:白及多糖能显著促进糖尿病溃疡创面愈合(与PBS组比较P＜0.05),病理组织学检查发现白及多糖能有效刺激炎症细胞浸润,促进上皮组织形成,FB增殖明显,OHP含量增加.结论:白及多糖可能通过促进炎症细胞浸润、增加OHP含量的合成和释放、FB的增殖,从而促进糖尿病溃疡创面愈合.
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| 关 键 词： | 糖尿病溃疡 白及多糖 |
| 收稿时间： | 2010/12/29 0:00:00 |
Diabetes mellitus ulcers treatment with Bletilla striata polysaccharide |
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| YU Linhu,NIE Xuqiang,PAN Huijun,LING Shuang,ZHANG Dandan and BIAN Ka.Diabetes mellitus ulcers treatment with Bletilla striata polysaccharide[J].China Journal of Chinese Materia Medica,2011,36(11):1487-1491. |
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| Authors: | YU Linhu NIE Xuqiang PAN Huijun LING Shuang ZHANG Dandan and BIAN Ka |
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| Institution: | Murad Research Institute for Modernized Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;Murad Research Institute for Modernized Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;Murad Research Institute for Modernized Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;Murad Research Institute for Modernized Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;Murad Research Institute for Modernized Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;Murad Research Institute for Modernized Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;E-Institute of Shanghai University, Division of Nitric Oxide and Inflammatory Medicine, Shanghai 201203, China |
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| Abstract: | The aim of this study was to evaluate the efficacy of Bletilla striata polysaccharide on diabetes mellitus ulcers. Diabetes mellitus animal model was established by single ip injection of streptozotocin (STZ, 50 mg x kg(-1)) with the criteria of blood glucose > or = 16.7 mmol x L(-1) after 72 h. 4 weeks after STZ injection, each animal received two full thickness incisional wounds (1.8 cm in diameter). The wounds then were divided into B. striata polysaccharide group and PBS group. Wound closure rate, fibroblast (FB) infiltration, hydroxyproline (OHP) content and myeloperoxidase (MPO) levels were examined on day 3, 7, 14, 21 post wound. The treatment of B. striata polysaccharide significantly facilitated diabetes mellitus ulcers healing compared to PBS group. Histological analysis showed that B. striata polysaccharide markedly increased inflammatory cell infiltration in wound area. The herb also strongly evaluation of FB, OHP demonstrated a significantly increased in B. striata polysaccharide group. B. striata polysaccharide group promoted wound closure by means of enhanced inflammatory cell infiltration and re-epithelialization, and the promotion of FB and OHP levels. |
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| Keywords: | diabetes mellitus ulcers Bletilla striata polysaccharide |
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