|
|||||
|
|
| Ad-hBMP-2转染体外培养人退变腰椎间盘细胞的研究 | |
| 引用本文： | 顾海伦,吕刚,李建军,刘莉,黄涛,原泉. Ad-hBMP-2转染体外培养人退变腰椎间盘细胞的研究[J]. 中国矫形外科杂志, 2005, 13(17): 1331-1333 |
| 作者姓名： | 顾海伦 吕刚 李建军 刘莉 黄涛 原泉 |
| 作者单位： | 1. 中国医科大学第一临床学院骨科,沈阳,110001 2. 沈阳中国医科大学第一临床学院骨科 3. 中国医科大学第二临床学院骨科 4. 中国医科大学公共卫生学院营养与食品卫生教研室 |
| 摘 要： | [目的]研究人骨形态发牛蛋白-2腺病毒表达载体（Ad—hBMP-2）转染体外培养人退变腰椎问盘细胞，分析其对腰椎间盘细胞影响.[方法]成人退变腰椎间盘细胞体外培养，通过免疫组化和染色体分析鉴定椎间盘细胞。利用免疫荧光和蛋白印迹法检测不同转染剂量对细胞BMP-2表达的影响。[结果]体外培养成人退变腰椎间盘细胞第2代鉴定具有其结构功能。转染后通过免疫荧光和蛋白印迹法可见随转染剂量增加BMP-2表达量逐渐增加。[结论]Ad—hBMP-2可高效转染体外培养人退变椎间盘细胞，同时随转染剂量增加BMP-2表达量逐渐增加。 |
| 关 键 词： | 腺病毒 骨形态蛋白2 椎间盘细胞 基因转染 退变椎间盘细胞 退变腰椎间盘 细胞体外培养 转染 hBMP-2 蛋白印迹法 |
| 收稿时间： | 2005-03-28 |
| 修稿时间： | 2005-05-09 |
Adenovirus-mediated human bone morphogenetic protein 2 gene transfer to human intervertebral disc cells and related research |
|
| Gu HaiLun;Lv Gang;Li JianJun;Liu Li;Huang Tao;Yuan Quan. Adenovirus-mediated human bone morphogenetic protein 2 gene transfer to human intervertebral disc cells and related research[J]. The Orthopedic Journal of China, 2005, 13(17): 1331-1333 | |
| Authors: | Gu HaiLun Lv Gang Li JianJun Liu Li Huang Tao Yuan Quan |
| Abstract: | [ Objective] To study the effects of Adenovirus-mediated human bone morphogenetic protein 2 gene transter to human intervertebral disc cells in vitro. [Method] Human intervertebral disc cells are cultured in vitro and identified by immunohistochemical staining and chromosome analysis. The expression of hBMP-2 in these cells after infection with different doses was determined by immunofiuorescence and Western blot analysis. [ Result] The second generation human intervertebral disc cells were confirmed. The expression of hBMP-2 in these cells was gradually increased after transfection with different dose. [ Conclusion] Ad-BMP-2 can infect human intervertebral disc cells with high efficiency and the expression of hBMP-2 after infection is dose dependent. |
| Keywords: | Adenoviral vector Bone morphogenetic Intervertebral disc cells Gene transfection |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |