塞来昔布对嘌呤氨基核苷诱导的足细胞凋亡中COX-2表达水平的影响

目的:旨在通过特异性环氧合酶-2(COX-2)抑制剂塞来昔布对嘌呤氨基核苷诱导的足细胞凋亡中足细胞COX-2表达水平变化的影响,初步探讨特异性COX-2抑制剂对足细胞保护作用的机制。方法:以条件永生性小鼠足细胞为研究对象,分为6组:正常对照组(CON)、嘌呤霉素氨基核苷组(PA)、地塞米松组(DEX)、塞来昔布组(CELE)、地塞米松+嘌呤氨基核苷组(DEX+PA)、塞来昔布+嘌呤氨基核苷组(CELE+PA),分别于0h、8h、24h和48h四个时间点用Hoechst 33258染色检测足细胞凋亡率,24h抽提蛋白用于Western blot检测COX-2的蛋白表达。结果:PA组与正常对照组相比细胞凋亡率明显增多(P<0.001),DEX+PA、CELE+PA组与PA组比较细胞凋亡率明显减少(P<0.05);正常对照组表达COX-2,用PA诱导凋亡后,24h时COX-2表达明显增加,用CELE、DEX干预后COX-2表达水平有所减少(P<0.05)。结论:特异性COX-2抑制剂CELE可以减少PA诱导的足细胞的凋亡率;CELE抑制PA诱导的足细胞凋亡的作用机制可能与CELE对足细胞所表达的COX-2水平变化的影响有一定的相关性。

Effect of Celecoxib on COX-2 Expression of Podocyte Apoptosis Induced by Puromycin Amino-nucleoside

Objective：To investigate the direct effect of celecoxib,a specific COX-2 inhibitor,on COX-2 expression of podocyte apoptosis induced by puromycin amino-nucleoside（PA） and establish the basis for studying the protective effects of specific COX-2 inhibitors on podocyte.Methods：The conditionally immortalized mouse podocytes were studied and divided into six groups：Control（CON）,puromycin aminonucleoside（PA）,dexamethasone（DEX）,celecoxib（CELE）,dexamethasone＋puromycin aminonucleoside（DEX＋PA） celecoxib ＋ puromycin aminonucleoside（CELE＋PA）.Apoptosis was measured by staining with Hoechst 33258 at 0h,8h,24h,48h;Western blot analysis was performed to measure the protein levels of COX-2 at 24h.Results：The percentage of apoptotic cells significantly increased in the podocytes that were exposed to PA compared with control group.In contrast,both dexamethasone and celecoxib significantly reduced PA-induced apoptosis（P0.05）.COX-2 expression was seen in control group.It was elevated profoundly after 24 hours induced by PA and reduced by use of DEX and CELE（P0.05）.Conclusion：Celecoxib prevents podocyte apoptosis induced by PA.The preventing effect of celecoxib on podocyte apoptosis may be potential interactions with COX-2 expression.