姜黄素在急性髓性白血病HL-60细胞中对凋亡调控蛋白Bax、Bak、Mcl-1的影响

为了探讨姜黄素在诱导急性髓性白血病HL-60细胞凋亡时，Bcl-2基因家族成员是否参与了其调控过程。选用Bcl-2基因家族成员Mcl-1、Bax、Bak蛋白为研究对象，SABC法检测其表达状况；用流式细胞术测定DNA直方图中Sub-G1峰值、末端TdT酶标技术检测TUNEL细胞阳性率。发现当25μmol/L姜黄素分别处理HL-60细胞24h、36h、48h后，可使Mcl-1表达下调，Bax和Bak上调，呈时间依赖性。在各处理组，Mcl-1、Bax、Bak表达同对照组相比有显著差异(F值分别为3.443、8.328；P值分别为0.040、0.001)。结果表明Bcl-2基因家族成员确实参与了姜黄素诱导HL-60细胞凋亡的调控过程。

The Study of the Effect of Curcumin on Apoptosis-Regulating ProteinMcl-1, Bax and Bak in Acute Myeloid Leukemia HL-60 Cell Line

Whether or not the Bcl-2 gene family was involved in modulating mechanism of apoptosis induced by curcumin in acute myeloid leukemia HL-60 cell line was investigated. The Bcl-2 family members Mcl-1, Bax and Bak were selected as subjects. The expression of these proteins was detected by using SABC method. The attitude of Sub-G1 peak in DNA histogram was determined by FCM. The TUNEL positive cell percentage was identified by terminal deoxynucleotidyl transferase (TdT)-mediated Biotin dUNP end labeling technique. It was found that after treatment of HL-60 cells with 25 μmol/L curcumin for 24 h, 36 h, 48 h, respectively, the expression level of Mcl-1 was down-regulated and that of Bax up-regulated in a time-dependent manner. In different treated groups, the expression levels of Mcl-1, Bax and Bak were significantly different from that of control group (F values were 3.443 and 8.328, respectively and P values were 0.040 and 0.001 respectively ). It was suggested that the Bcl-2 gene family indeed participated in the regulatory process of apoptosis induced by curcumin in HL-60 cells.