| 参芎注射液对大鼠脑缺血再灌注后神经细胞凋亡的相关研究 |
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| 引用本文： | 张智博,陈本阳,唐璇,彭旭,唐璐. 参芎注射液对大鼠脑缺血再灌注后神经细胞凋亡的相关研究[J]. 中华老年心脑血管病杂志, 2008, 10(10): 775-778 |
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| 作者姓名： | 张智博 陈本阳 唐璇 彭旭 唐璐 |
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| 作者单位： | 1. 长沙市第一医院神经内科,长沙,410005
2. 南华大学内科教研室 |
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| 摘 要： | 目的探讨参芎注射液对大鼠脑缺血再灌注后神经细胞凋亡及凋亡相关因子caspase-8、抗凋亡因子FLIP的蛋白及其mRNA表达的影响。方法选择SD大鼠100只随机分为正常组(10只)、假手术组(10只)、模型组(40只)和参芎注射液治疗组(干预组,40只),采用TUNEL法检测神经细胞凋亡,免疫组织化学及RT-PCR法分别检测caspase-8、FLIP的蛋白和mRNA表达。结果与正常组和假手术组比较,模型组大鼠各时间点凋亡细胞明显增加(P<0.01),caspase-8、FLIP的蛋白和mRNA表达明显增强(P<0.05,P<0.01)。与同时间点模型组比较,干预组大鼠凋亡细胞明显减少,caspase-8蛋白和mRNA表达明显减弱(P<0.05,P<0.01),FLIP蛋白和mRNA表达分别于再灌注6 h和12 h时间点明显增强(P<0.05)。结论参芎注射液能通过减弱caspase-8表达及增强FLIP表达,从而拮抗大鼠脑缺血再灌注后神经细胞凋亡。
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| 关 键 词： | 脑缺血 再灌注 细胞凋亡 参芎注射液 半胱氨酸天冬氨酸蛋白酶 |
The relative study on the effect of Shenxiong injection on the neuronal apoptosis in rat after cerebral ischemia-reperfusion |
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| ZHANG Zhi-bo,CHEN Ben-yang,TANG Xuan,et al. The relative study on the effect of Shenxiong injection on the neuronal apoptosis in rat after cerebral ischemia-reperfusion[J]. Chinese Journal of Geriatric Cardiovascular and Cerebrovascular Diseases, 2008, 10(10): 775-778 |
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| Authors: | ZHANG Zhi-bo CHEN Ben-yang TANG Xuan et al |
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| Affiliation: | ZHANG Zhi-bo,CHEN Ben-yang,TANG Xuan,et al(Department of Neurology,The First Hospitol of Changsha,Changsha 410005,China) |
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| Abstract: | Objective To study the effect of Shenxiong injection on the neuronal apoptosis and the expression of the protein and mRNA of caspase-8 and FLIP in rat after cerebral ischemia-reperfusion.Methods 100 healthy male SD rats were randomly divided into 4 groups:normal group(n=10),sham-operated group(n=10),model group(n=40),Shenxiong injection treated group(treated group,n=40).A model of cerebral middle artery occlusion in rat was performed with intraluminal filament occlusion.The neuronal apoptosis was detected b... |
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| Keywords: | brain ischemia reperfusion apoptosis Shenxiong injection caspases |
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