| 庚型肝炎病毒NS5非结构蛋白在昆虫细胞中的表达 |
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| 引用本文： | 任浩,朱分禄,朱诗应,宋燕斌,戚中田. 庚型肝炎病毒NS5非结构蛋白在昆虫细胞中的表达[J]. 第二军医大学学报, 2000, 21(1): 16-19 |
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| 作者姓名： | 任浩 朱分禄 朱诗应 宋燕斌 戚中田 |
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| 作者单位： | 第二军医大学基础医学部微生物学教研室,上海,200433 |
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| 基金项目： | 国家自然科学基金!资助项目（39680018） |
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| 摘 要： | 目的;研制特异的抗ＧＢＶ－Ｃ／ＨＧＶ抗体诊断试剂。方法：ＰＣＲ扩增ＧＢＶ－Ｃ／ＨＧＶ ＮＳ５基因片段并定向克隆至转座载体ｐＦａｓｔＢａｃ ＨＴａ,转化ＤＨ１０Ｂａｃ感受态细胞,３７℃振荡培养４ｈ使发生转座,于三抗选择平皿筛选重组ｂａｃｍｉｄ。脂质体介导转染ｓｆ９细胞,将转染上清再次感染ｓｆ细胞,以批量表达ＮＳ５重组蛋白。利用ＳＤＳ－ＰＡＧＥ和Ｗｅｓｔｅｒｎ ｂｌｏｔ方法分析,检测重组蛋白。结果：序
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| 关 键 词： | NS5蛋白 庚型肝炎 GBV－C／HGV 基因表达 |
| 文章编号： | 0258-879X(2000)01-0016-04 |
| 修稿时间： | 1999-08-02 |
Expression of GBV-C/HGV NS5 non-structure protein in insect cells |
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| REN Hao,ZHU Fen-Lu,ZHU Shi-Ying,SONG Yan-Bin,QI Zhong-Tian. Expression of GBV-C/HGV NS5 non-structure protein in insect cells[J]. Former Academic Journal of Second Military Medical University, 2000, 21(1): 16-19 |
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| Authors: | REN Hao ZHU Fen-Lu ZHU Shi-Ying SONG Yan-Bin QI Zhong-Tian |
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| Affiliation: | REN Hao(Department of Microbiology, Department of Basic Medicine, Second Military MedicalUniversity, Shanghai 200433, China)ZHU Fen-Lu(Department of Microbiology, Department of Basic Medicine, Second Military MedicalUniversity, Shanghai 200433, China)ZHU Shi-Ying(Department of Microbiology, Department of Basic Medicine, Second Military MedicalUniversity, Shanghai 200433, China)SONG Yan-Bin(Department of Microbiology, Department of Basic Medicine, Second Military MedicalUniversity, Shanghai 200433, China)QI Zhong-Tian(Department of Microbiology, Department of Basic Medicine, Second Military MedicalUniversity, Shanghai 200433, China) |
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| Abstract: | |
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| Keywords: | hepatitis G virus NS5 protein expression |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
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