羊水细胞培养成功与失败结果分析

目的探讨改善羊水细胞培养成功率的条件。方法在B超引导下经羊膜腔穿刺,抽取羊水,接种于斜面培养管中,37℃5%CO2培养箱中静止培养,用胰酶消化法收获细胞,常规G显带,进行核型分析。结果通过控制影响羊水培养的各个环节,改善细胞培养环境,缩短了羊水培养的时间,提高了羊水培养的成功率,增加了可供分析染色体核型。结论及时、正确处理羊水标本以及控制影响细胞培养成功的各个环节,准确判断羊水收获时机,可提高羊水细胞培养的成功率。

THE EXPERIENCE OF SUCCESS OR FAILURE ON AMNIOTIC FLUID CELL CULTURE

Objective To study and improve the success rate of cell culture conditions of amniotic fluid.Methods The amniotic fluid was extracted by the B ultrasound guided in amniocentesis,then was inoculated in the slant pipe,37℃ 5%CO2 incubator in static culture,and cells were harvested by trypsin digestion method,conventional G banding,carries on the analysis of the karyotype.Results Through the control effect of amniotic fluid culture of each link,improved cell culture environment,shorten the time of amniotic fluid culture,improve the success rate of amniotic fluid culture,increases the available analysis of chromosome karyotype.Conclusion Timely and correctly handle the amniotic fluid specimens and control effects of cell culture success of each link,accurate judgement of the amniotic fluid harvest time,can improve the success rate of amniotic fluid cell culture.