汉防己甲素滴眼液防治大鼠角膜移植排斥反应的实验研究

目的:探讨汉防己甲素滴眼液防治角膜移植排斥反应效果及最佳用药浓度。方法:建立大鼠角膜移植排斥反应模型,随机分5组:A、B、C、D、E组为SD-Wistar大鼠行同种异体角膜移植术,SD鼠为受体,Wistar鼠为供体,其中A组为空白对照组,B～D组分别为0.3%、0.5%、1.0%汉防己甲素滴眼液治疗组,E组为0.1%地塞米松治疗组。术后不同时间裂隙灯显微镜记录及比较各组角膜植片新生血管情况,并观察植片病理学改变。结果:植片内主要为淋巴细胞及巨噬细胞浸润。B、C、D组术后7d、14d、21d、28d时角膜植片水肿、淋巴细胞及巨噬细胞浸润及新生血管明显少于A组(P<0.05)。术后2周不同Tet浓度组间比较C组排斥指数最低,与E组无统计学差异(P>0.05)。结论:0.5%浓度汉防己甲素滴眼液可有效抑制角膜移植排斥反应。

An Experimental Study on the Inhibition Effect of Corneal Allograft Rejection in Rat by Local Application of Tetrandrine Eye Drops

Purpose: To investigate the effect and the best concentration of Tetrandrine (Tet) eye drops in inhibition of the corneal allograft rejection in rat. Methods: Eighty SD rats were randomly divided for being treated with 0.3%?0.5%?1.0% Tetrandrine eye drops, respectively. 0.1% Dexamethasone eye drops-treated and control groups. After the rat model of heterograft-heterotopic graft rejection was made, slit-limp microscope and HE staining, light microscope and microphoto-analyses technique were used to observe the neovascular formation and inflammation at different time points at post-operation. Results: The types of the cells in the surgical area were mainly lymphocytes and mononuclear-macrophage. Corneal neovascularization and inflammatory cells were significantly inhibited in 0.5% Tetrandrine eye drops-treated group (P < 0.05), compared with control group at 7 d?14 d?21 d?28 d post-operation. Conclusions: The results indicate that 0.5% Tet eye drops significantly inhibit the corneal allograft rejection in rats without obvious side effects.