应激性胃溃疡大鼠血清铜、锌水平及SOD活性和GSH含量的性别差异研究

目的探讨应激性胃溃疡的性别差异及其可能的机制。方法采用冷冻-束缚方法建立应激性胃溃疡大鼠模型，并测定血清铜、锌水平及超氧化物岐化酶（SOD）活性、还原型谷胱甘肽（GSH）含量。结果经冷应激后，雄性大鼠胃溃疡指数明显高于雌性大鼠（p〈0．05）；与室温对照组相比较，应激后各组血清锌水平升高、铜水平下降，且雄性大鼠血清铜水平下降幅度明显大于雌性大鼠（P〈0．05）；雌性和雄性组血清中SOD活性均极明显地降低（P〈0．01），且雄性大鼠血清SOD活性下降幅度极显著大于雌性大鼠（P〈0．01）。血清GSH含量均极明显地升高（P〈0．01）；结论冷应激促使铜、锌在体内重新分布，性别不同对大鼠应激性胃溃疡的发生及其血清铜水平和SOD活性具有一定的影响。

Some Gender Difference on Stress Gastric Ulcer in Rats

Objective To investigate the gender difference and potential mechanism of stress gastric ulcer in rats. Methods Fourty male and 40 female SD rats about 200-220 g were randomly divided into cold stress group (20 rats) and room temperature control group (20 rats) according to gender respectively. Rats of cold stress groups were afflicted gastric ulcer by cold stress method. Then the gastric ulcer index was compared between cold stress group of male and female groups, meanwhile the concentration of serum copper and zinc, activity of superoxide dismutase (SOD), and content of reduced glutathione (GSH) in every group were determined and analyzed. Results The gastric ulcer index in male group was significant higher than that in female group. Concentration of serum zinc upgraded and serum copper decreased after cold stress (P<0.05 or P<0.01) and the serum copper of male group was more reduced(P<0.05) . Activity of SOD in serum of both genders were decreased significantly (P<0.01), and the male group was even more reduced (P<0.01). Content of GSH in serum of both genders were increased overtly (P<0.01). Conclusion The rat's gender have some effects on gastric ulcer of rats. Cold stress provokes copper and zinc re-distribution in vivo and free radical cleaning system differential priming response in two genders are part of the possible inherent mechanism of sexual difference in stress gastric ulcer.