| 屋尘螨注射液的体外生物活性 |
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| 引用本文： | 祝戎飞,黄南,郭其苗,祁姗姗,张威,李文静,陈欢,刘光辉.屋尘螨注射液的体外生物活性[J].中国医院药学杂志,2011(7). |
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| 作者姓名： | 祝戎飞 黄南 郭其苗 祁姗姗 张威 李文静 陈欢 刘光辉 |
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| 作者单位： | 华中科技大学同济医学院附属同济医院过敏反应科; |
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| 摘 要： | 目的:探讨屋尘螨注射液的体外生物活性及不同批次注射液的相对稳定性。方法:收集60例单纯尘螨过敏的哮喘患者血清,制备屋尘螨血清池,采用放射免疫吸附(RAST)抑制试验方法测定屋尘螨注射液体外生物活性,并比较不同批次间的稳定性;同时采用聚丙烯酰胺凝胶电泳法比较不同批次屋尘螨注射液蛋白组分有无差异;酶联免疫吸附法(ELISA)测定屋尘螨注射液主要致敏蛋白Der p1的含量。结果:体外RAST抑制试验显示屋尘螨注射液能抑制标准屋尘螨抗原与IgE的特异性结合,具有很强的生物活性,3种批次的屋尘螨注射液抑制试验结果相似,稳定性良好;电泳结果提示3种批次的屋尘螨注射液蛋白组分相同,具有良好的一致性;ELISA法检查发现3种批次的屋尘螨注射液主要致敏蛋白Der p1含量相同。结论:屋尘螨注射液具有较强的体外生物活性,并且批次间稳定性好。
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| 关 键 词： | 屋尘螨 放射免疫吸附抑制试验 生物活性 |
Bioactivity of dermatophagoides pteronyssinus solution in vitro |
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| ZHU Rong-fei,HUANG Nan,GUO Qi-miao,QI Shan-shan,ZHANG Wei,LI Wen-jing,CHEN Huan,LIU Guang-hui.Bioactivity of dermatophagoides pteronyssinus solution in vitro[J].Chinese Journal of Hospital Pharmacy,2011(7). |
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| Authors: | ZHU Rong-fei HUANG Nan GUO Qi-miao QI Shan-shan ZHANG Wei LI Wen-jing CHEN Huan LIU Guang-hui |
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| Institution: | ZHU Rong-fei,HUANG Nan,GUO Qi-miao,QI Shan-shan,ZHANG Wei,LI Wen-jing,CHEN Huan,LIU Guang-hui(Department of Allergy,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Hubei Wuhan 430030,China) |
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| Abstract: | OBJECTIVE To investigate the bioactivity of dermatophagoides pteronyssinus solution and the relative stability of different batches in vitro.METHODS 60 dust-mite allergic asthma patients' sera were collected to prepare an allergic sera pool.Radioallergosorbent test inhibition was performed to test bioactivity in vitro.The relative stabilities of different batches were compared.Polyacrylamide gel electrophoresis(PAGE) was used to compare the protein ingredients of different batches of Dermatophagoides pteron... |
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| Keywords: | Dermatophagoides pteronyssinus RAST Inhibition bioactivity |
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