组织芯片和免疫组化技术在子宫内膜腺癌诊断的应用

目的评价组织芯片和免疫组化技术在子宫内膜腺癌诊断中的应用价值。方法使用组织芯片和免疫组化技术,观察60例子宫内膜腺癌和30例正常子宫内膜的P16、PTEN、E-cadherin和CtBP1表达情况。结果P16、PTEN、E-cadherin在子宫内膜癌的阳性率显著高于正常子宫内膜（P〈0.05）,而CtBP1的表达差异无统计学意义（P〉0.05）;4个免疫组化指标在不同分化癌细胞的表达差异均无统计学意义（P〉0.05）。结论P16、PTEN和E-cadherin与子宫内膜腺癌有关,对于病理诊断有参考价值。使用组织芯片和免疫组化技术能够对批量样本进行快速检测。

Application of Tissue Microarray and Immunohistochemistry Technique to the Diagnosis of Endometrial Cancer

Objective To evaluate the significance of the appliance of tissue microarray and immunohistochemistry technique on detecting the gene expression in endometrial cancer.Methods The expressions of P16,PTEN,E-cadherin and CtBP1 of the 60 patients with endometrial cancer（experimental group） and 30 patients with normal endometrial（control group） were observed by tissue microarray technology and immunohistochemichal methods.Results The Positive expressions of P16,PTEN and E-cadherin of the patients in the experimental group were significantly higher than those in the control group（P 0.05）,while there was no difference of CtBP1 expression between the two groups（P 0.05） .Besides,there were no differences of the expressions of 4 indicators of immunohistochemistry in different cancer cell differentiation（P 0.05） .Conclusion Tissue microarray and immunohistochemistry technique could be useful and reliable in the quick diagnosis of endometrial cancer in the mass samples,which indicates that there are relationships between P16,PTEN,E-cadherin and endometrial cancer and they are of reference values for pathologic diagnosis.